NCK1 monoclonal antibody

• Catalog number
  11-711-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Mouse Monoclonal Antibody against Human NCK1, Clone: EM-06
• Antigen
  NCK1
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  EM-06
• Applications
  WB, ICC
• Immunogen
  Purified recombinant second SH3 domain of human Nck1 fused to GST
• Format conjugation
  purified; unconjugated
• Specificity
  The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.
• Research area
  Adaptor Proteins (Rodent), Adaptor Proteins (Human)
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Mouse Monoclonal Antibody against Human NCK1, Clone: EM-06 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  *Borroto A, Arellano I, Dopfer EP, Prouza M, Suchánek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1;190(3):1103-12.
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  NCK1   monoclonal  
• Gene symbol
  NCK1-DT, NCK1
• Short name
  Anti-NCK1 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to NCK1
• Alternative technique
  antibodies
• Alternative to gene target
  NCK adaptor protein 1, NCK and nck-1 and NCKalpha, NCK1 and IDBG-58017 and ENSG00000158092 and 4690, receptor tyrosine kinase binding, nuclei, Nck1 and IDBG-193200 and ENSMUSG00000032475 and 17973, NCK1 and IDBG-638662 and ENSBTAG00000018427 and 536154

Gene info

• Identity
  HGNC:49645
• Gene
  NCK1-DT
• Long gene name
  NCK1 divergent transcript
• Synonyms gene
  • NCK1-AS1
• Synonyms gene name
  • NCK1 antisense RNA 1 (head to head)
• Synonyms
  • SLC35G2-AS1
• Synonyms name
  • SLC35G2 antisense RNA 1
• Locus
  3q22.3
• Discovery year
  2014-01-28
• Entrez gene record
  101927597
• Pubmed identfication
  • 29416014
• RefSeq identity
  • NR_110175
• Classification
  • Divergent transcripts
• VEGA ID
  OTTHUMG00000159786

Gene info

• Identity
  HGNC:7664
• Gene
  NCK1
• Long gene name
  NCK adaptor protein 1
• Synonyms gene
  • NCK
• Synonyms
  • NCKalpha
• GenBank acession
  • X17576
• Locus
  3q22.3
• Discovery year
  1998-11-04
• Entrez gene record
  4690
• Pubmed identfication
  • 7806213
  • 9737977
• RefSeq identity
  • NM_006153
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000159781

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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