Rat Brain antibody Control Lysate
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Catalog number
X1030
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Price
Please ask
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Size
100uL
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Category
Other Reagents
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Long description
Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls for antibodies to proteins expressed in these cells. The mouse fibroblast cell line 3T3 and its RSV-transformed counterpart serve as negative and positive controls, respectively, for antibodies to proteins that are phosphorylated as a result of transformation by RSV (Rous sarcoma virus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues of neuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain. At right is an example of the use of positive and negative control Iysates in western blotting. The blot of 3T3 cell Iysate (lane 1) and RSV-transformed 3T3 (lane 2) was probed with anti-phosphotyrosine peroxidase conjugate to reveal those proteins that are tyrosine-phosphorylated in response to transformation by
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Antibody come from
n/a
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Other description
Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
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Clone
not specified
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Antigen antibody binding interaction
Rat Brain antibody Control Lysate
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Antibody is raised in
see techfile
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Optimal concentration should be evaluated by serial dilutions. Suggested use: A volume of 10-25ul is suggested for immunobloting.
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Storage
-20ºC
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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French translation
anticorps
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Gene target
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Short name
Brain antibody Control Lysate
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Technique
Antibody, Control, antibodies against human proteins, antibodies for, controls
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Host
Rat
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Isotype
not specified
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Label
unlabeled
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Species
Rat, Rats
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Alternative name
Rat Brain (antibody to-) reference Lysate
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Alternative technique
antibodies, controls
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Tissue
brain, control, lysate
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Virus
lysate
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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