Recombinant Human PBEF / Visfatin / NAMPT Protein (His & GST Tag)
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Catalog number
PKSH031981-20µg
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Price
Please ask
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Size
20µg
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Synonym
1110035O14Rik;PBEF;PBEF1;VF;VISFATIN
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Activity
NA
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Sequence
Met 1-His 491
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Fusion tag
N-His & GST
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Accession
P43490
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Expressed Host
Baculovirus-Insect Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
＞90 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
83.3 kDa
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AP Mol Mass
75 kDa
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Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 0.3mM DTT 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements.
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Background
Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme belonging to the family of glycosyltransferases, to be specific, the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1- pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells, as well as plasma NAMPT and NAD+ levels, were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NAMPT, MGST3
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Short name
Recombinant PBEF / Visfatin / NAMPT Protein (His & GST Tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens PBEF / Visfatin / nicotinamide phosphoribosyltransferase Protein (histidine & GST detection labelled)
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Alternative technique
rec
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Alternative to gene target
nicotinamide phosphoribosyltransferase, 1110035O14Rik and PBEF and PBEF1 and VF and VISFATIN, NAMPT and IDBG-35259 and ENSG00000105835 and 10135, nicotinamide phosphoribosyltransferase activity, nuclei, Nampt and IDBG-134049 and ENSMUSG00000020572 and 59027, BT.33608 and IDBG-632034 and ENSBTAG00000015509 and 520472
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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