NAMPT Antibody / Visfatin
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Catalog number
R32446
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
NAMPT / Visfatin
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P, ELISA (protein)
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml,ELISA: 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
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Added buffer
Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
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Intented use
This Visfatin antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P43490
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Purity
Antigen affinity
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Description
Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
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Immunogen
Amino acids L62-N337 were used as the immunogen for the Visfatin antibody.
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Storage
Prior to reconstitution, store at 4oC. After reconstitution, the Visfatin antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Localization
Predominantly cytoplasmic, some nuclear staining may also be seen
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
NAMPT
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Short name
Anti- NAMPT / Visfatin
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to NAMPT / Visfatin
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Alternative technique
antibodies
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Alternative to gene target
nicotinamide phosphoribosyltransferase, 1110035O14Rik and PBEF and PBEF1 and VF and VISFATIN, NAMPT and IDBG-35259 and ENSG00000105835 and 10135, nicotinamide phosphoribosyltransferase activity, nuclei, Nampt and IDBG-134049 and ENSMUSG00000020572 and 59027, BT.33608 and IDBG-632034 and ENSBTAG00000015509 and 520472
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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