Medium 199 (For Suspension Culture) With Earle's salts, L-Glutamine and Sodium bicarbonate Without Calcium chloride and Magnesium sulphate

  • Catalog number
    AL043A-500ML
  • Price
    Please ask
  • Size
    500ML
  • Description
    Medium 199 (For Suspension Culture) With Earle's salts, L-Glutamine and Sodium bicarbonate Without Calcium chloride and Magnesium sulphate is an orangish red, clear solution. It is used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
  • Shelf life
    12 months.
  • Storage
    Store at 2-8°C
  • Appearance
    Orangish red colored, clear solution.
  • PH
    7.00 -7.60
  • Osmolality in mOsm Kg H2O
    280.00 -320.00
  • Sterility
    No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
  • Cultural Response
    The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
  • Endotoxin Content
    NMT 5EU/ml
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Ordering
    To order Medium 199 (For Suspension Culture) With Earle's salts, L-Glutamine and Sodium bicarbonate Without Calcium chloride and Magnesium sulphate, please use the Cat. Nr.AL043A-500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Medium 199 (For Suspension Culture) With Earle's salts, L-Glutamine and Sodium bicarbonate Without Calcium chloride and Magnesium sulphate
  • Additional description
    There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Group
    media
  • Gene target
  • Short name
    Medium 199 (For Suspension Culture) With Earle's salts, L-Glutamine Sodium bicarbonate Without Calcium chloride Magnesium sulphate
  • Technique
    culture, Medium, cell culture Media
  • Alternative name
    Medium 199 (to measure Suspension Culture) including Earle's salts, L-Glutamine and Sodium bicarbonate Without Calcium chloride and Magnesium sulphate
  • Alternative technique
    cellculture
  • Virus
    hhv
MeSH Data
  • Name
  • Concept
    Scope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
  • Tree numbers
    • E05.481.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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