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Available ordering format
Lyophilized
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Immunogen
Native, NAD-ME purified from mitochondria isolated from mature amaranth leaves.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum + 0.01% sodium azide
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL), immunoprecipiatation (IP)
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Connected products
collection of antibodies to proteins involved in photosynthesiscollection of antibodies to mitochondrial proteinsPlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 (WB), 1: 25 to 1: 100 (IL)
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Molecular weight expected аpparent
65 kDa
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Verified reactivity
Amaranthus hypochondriacus, Arabidopsis thaliana, Flaveria sp., Nicotiana tabacum, Zea mays
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Possible reactivity
Amborella trichopoda, Arabis alpina, Beta vulgaris, Brachypodium distachyon, Brassica oleracea, Citrus sinensis, Coffea canephora, Cucumis sativus , Cynara cardunculus, Erythranthe guttata, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium raimondii, Hordeum vulgare, Jatropha curcas, Malus domestica, Medicago truncatula, Morus notabilis, Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Prunus persica, Ricinus communis, Selaginella moellendorffii, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Theobroma cacao, Triticum aestivum, Zostera marina, Wollemia nobilis, Vitis vinifera
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No reactivity
No confirmed exceptions from predicted reactivity are currently known.
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Supplementary information
This antibody recognizes the large subunit (MEL) of the NAD-ME.Immunolocalization method is described in Long et al. (1994).
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References
Long et al. (1994). Cloning and analysis of the C4 photosynthetic NAD-dependent malic enzyme of amaranth mitochondria. J Biol Chem. 1994 Jan 28;269(4):2827-33.
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Scientific context
NAD-ME (Mitochondrial NAD-dependent malic enzyme) is an enzyme which in some C4 plants catalyzes the decarboxylation of 4 carbon malate in the bundle sheath cells, releasing carbon dioxide. This enzyme plays a key role in photosynthetic carbon fixation.
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Notes
Antibody can be used in immunolocalization using immunogold TEM.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
