N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
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Catalog number
K822-100
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Price
Please ask
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Size
100 assays
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Description
The FIRST and ONLY kit on the market for Nicotinamide N-Methyltransferase (NNMT) Inhibitor Screening
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Summary
• Detection method- Fluorescence (Ex/Em = 392/482 nm) • Species reactivity- Mammalian • Application - Screening/studying/characterizing potential inhibitors of NNMT
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Detection Method
Fluorescence (Ex/Em = 392/482 nm)
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Species Reactivity
Mammalian
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Applications
Screening/studying/characterizing potential inhibitors of NNMT
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Features Benefits
• Rapid assay and simple protocol • Reliable test for screening potential inhibitors of NNMT • Includes Inhibitor Control, 1-Methylnicotinamide (MNA)
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Storage Conditions
-80°C
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Shipping Conditions
dry ice
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Shelf life
12 months
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Background
N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Biovision’s NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.
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Test
Biovision supplies other types of Assays as 1.
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Additional description
Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbol
NNMT
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Short name
N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
N′-Nicotinamide Methyltransferase (NNMT) suppressor detection test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Product images
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