Multi Fusion-Tagged recombinant Protein 52-Kda containing 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA
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Catalog number
MFPM52-R-40
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Price
Please ask
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Size
40 ug
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Stock availability
Available
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Category
Recombinant Protein
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Antibody type
N/A
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Antibody host
N/A
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Antibody conjugate
His-Tag
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Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
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Notes
The Multi Fusion-Tagged recombinant Protein 52-Kda containing 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, Flag, S-tag, HA, KT3, E2, Au1, Au5, 6xHis-tags) for ELISA is manufactured for Research Use Only or for diagnostics purposes.
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Conjugated
The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Conjugation
Flag
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Description
Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag. The kilo Daltons subunit weight of Multi -Tagged recombinant Protein 52- 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, , S-tag, HA, KT3, E2, Au1, Au5, -tags) for ELISA compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
HLA-T, IGHV3-71, GALNT7, ESB3, TBXT, PLS3, HLA-G, PIRC83, PIRC88
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Short name
Multi -Tagged recombinant Protein 52- 16-tags (T-7, HSV, C-myc, VSV-G, Glu-Glu, V5, e-tag, , S-tag, HA, KT3, E2, Au1, Au5, -tags) for ELISA
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Technique
Recombinant, ELISA, multi, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. multi, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. adi advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Label
tag
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Species
All species
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Alternative name
Multi Fusion-Tagged Rec. Protein 52-kiloDalton containing 16-tags (T-7, HSV, C-v-myc myelocytomatosis viral oncogene homolog (avian), VSV-G, L-gutamic acid-L-gutamic acid, V5, e-detection labelled, Flag, S-detection labelled, HA, KT3, E2, Au1, Au5, 6xHis-tags) to measure Enzyme-linked immunosorbent assay
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Alternative technique
rec, elisas
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Alternative to gene target
v-myc myelocytomatosis viral oncogene homolog (avian), bHLHe39 and c-Myc and MRTL and MYCC, MYC and IDBG-35442 and ENSG00000136997 and 4609, E-box binding, nuclei, Bmyc and IDBG-147082 and ENSMUSG00000049086 and 107771, MYC and IDBG-644147 and ENSBTAG00000008409 and 511077
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Virus
hsv
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Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
esterase B3
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Locus
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Discovery year
2001-06-22
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Pubmed identfication
Gene info
Gene info
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Identity
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Gene
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Long gene name
plastin 3
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1997-08-18
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Entrez gene record
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Pubmed identfication
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Classification
- EF-hand domain containing
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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