MTS Cell Proliferation Colorimetric Assay Kit

• Catalog number
  K300-500
• Price
  Please ask
• Size
  500 assays

• Description
  Sensitive, Non-radioactive Assay
• Summary
  • Detection- Absorbance (490-500 nm) • Applications: - Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients - Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals - Assessment of physiological mediators that inhibit cell growth
• Detection Method
  Absorbance (490-500 nm)
• Applications
  • Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. • Assessment of physiological mediators that inhibit cell growth.
• Sample Type
  • Adherent and suspension cells
• Features Benefits
  • Simple one-step procedure; takes ~ 4 hours • High-throughput • The entire assay can be performed directly in a 96-well plate and does not require washing/harvesting/or solubilization steps.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  BioVision’s MTS Cell Proliferation Assay Kit is a colorimetric method for sensitive quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the reduction of MTS tetrazolium compound by viable cells to generate a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells. The formazan dye produced by viable cells can be quantified by measuring the absorbance at 490-500 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. BioVision’s MTS assay is performed by adding the reagent directly into the cell culture media without the intermittent steps, which are required in the routine MTT assay. In addition, this high-throughput assay requires no washing or solubilization step and can be performed in 96-well microtiter plate.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  MTS   Proliferation   Kit  
• Gene symbol
  TIMM8A
• Short name
  MTS Proliferation Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  MTS cellular expantion colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

Gene info

• Identity
  HGNC:11817
• Gene
  TIMM8A
• Long gene name
  translocase of inner mitochondrial membrane 8A
• Synonyms gene
  • DFN1
• Synonyms gene name
  • translocase of inner mitochondrial membrane 8 (yeast) homolog A
  • translocase of inner mitochondrial membrane 8 homolog A (yeast)
• Synonyms
  • DDP
  • MTS
• GenBank acession
  • U66035
• Locus
  Xq22.1
• Discovery year
  1999-12-01
• Entrez gene record
  1678
• Pubmed identfication
  • 10552927
  • 8841189
• RefSeq identity
  • NM_004085
• VEGA ID
  OTTHUMG00000022028

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