MRP1, ABCC1, ABC transporter C family member 1
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Catalog numberAS09 472
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PricePlease ask
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Size100 µl
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Available ordering formatInquire
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ImmunogenKLH-conjugated synthetic peptide derived from Arabidopsis thaliana MRP1,Q9C8G9, At1g30400
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applicationsELISA (ELISA), western blot (WB)
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Connected productscollection of antibodies to tonoplast proteinsPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
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Molecular weight expected аpparent181.9 |180 kDa (Arabidopsis thaliana)
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Verified reactivityArabidopsis thaliana
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Possible reactivitydicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens
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No reactivityno confirmed exceptions from predicted reactivity known in the moment
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Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
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ReferencesGeisler et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.
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Scientific contextMRP1 (EC= 3.6.3.44) is a pump for glutathione S-conjugates. Alternative names: ABC transporter ABCC.1, multidrug resistance-associated protein 1, glutathione S-conjugate-transporting ATPase 1, ATP-energized glutathione S-conjugate pump 1
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Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for plant tonoplast membrane preparation is available here.
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Protein numberQ9C8G9
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TAIR numberAT1G30400
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Gene target
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Gene symbolABCC1, MSH3, ABCA3
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Short nameMRP1, ABCC1, ABC transporter C family member 1
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Alternative nameMRP1, ABCC1, ABC transporter C family member 1
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Gene info
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Identity
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Gene
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Long gene nameATP binding cassette subfamily C member 1
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Synonyms gene
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Synonyms gene name
- multidrug resistance associated protein 1
- ATP-binding cassette, sub-family C (CFTR/MRP), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ATP binding cassette subfamily C
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemutS homolog 3
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Synonyms gene name
- mutS (E. coli) homolog 3
- mutS homolog 3 (E. coli)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-09-28
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Entrez gene record
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RefSeq identity
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Classification
- MutS homologs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameATP binding cassette subfamily A member 3
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Synonyms gene
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Synonyms gene name
- ATP-binding cassette, sub-family A (ABC1), member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-08-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ATP binding cassette subfamily A
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VEGA ID