Mouse TRAIL-R4(Tumor Necrosis Factor Related Apoptosis Inducing Ligand Receptor 4) EIA Kit
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Catalog number
E-EL-M1213-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Mouse
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Target Synonym
NA
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRAIL-R4. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRAIL-R4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRAIL-R4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TRAIL-R4. You can calculate the concentration of Mouse TRAIL-R4 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.1 ng/mL
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Detection Range
0.156~10ng/mL
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Target Name
TRAIL-R4
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UNIProt ID
NA
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Mouse TRAIL-R4(Tumor Necrosis Factor Related Apoptosis Inducing Ligand Receptor 4) EIA Kit
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Type
Sandwich
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Description
This 1 is suited for programmed cell-death studies. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
TNFRSF10D, TNFSF10, TNFRSF10B
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Short name
Mouse TRAIL-R4(Tumor Necrosis Factor Apoptosis Ligand Receptor 4) EIA Kit
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Technique
apoptosis, Mouse, EIA, apoptosis pathway, eia, mouses
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Mouse TRAIL-R4(Tumor Necrosis Factor Related programmed cellular destruction Inducing Ligand Receptor 4) EIA reagent
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Alternative technique
celldeath, murine, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
tumor
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Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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