Mouse stem cell factor (mSCF)
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Catalog number
MBS400071
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Price
Please ask
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Size
0.05 mg
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Products_type
Active Protein
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Products_short_name
[stem cell factor]
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Other_names
[stem cell factor, partial; Kit ligand; kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor; KIT ligand; Mast cell growth factor; MGF]
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Products_gene_name
[mSCF]
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Other_gene_names
[KITLG; KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7; MGF; SCF; MGF; SCF; sKITLG]
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Purity
98.0% as determined by:(a) Analysis by RP-HPLC.(b) Anion-exchange FPLC.(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.(d) Gel filtration chromatography under non denaturing conditions.
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Form
White lyophilized (freeze-dried) powder.
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Storage_stability
Lyophilized rmSCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution rHuSCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Latin name
Mus musculus
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