Mouse SDF-1β(Stromal Cell Derived Factor 1 Beta) EIA Kit

• Catalog number
  E-EL-M1095-96T
• Price
  Please ask
• Size
  96Test

• Detection Type
  Colormetric
• Target Species
  Mouse
• Target Synonym
  NA
• Test principle
  This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SDF-1β. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SDF-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SDF-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SDF-1β. You can calculate the concentration of Mouse SDF-1β in the samples by comparing the OD of the samples to the standard curve.
• Sensitivity
  37.5 pg/mL
• Detection Range
  62.5~4000pg/mL
• Target Name
  SDF-1β
• UNIProt ID
  NA
• Tested Sample Types
  Serum, Plasma, Cell supernatant
• Product Name
  Mouse SDF-1β(Stromal Cell Derived Factor 1 Beta) EIA Kit
• Type
  Sandwich
• Technical data
• Standard Curve RUL
• Description
  For cells, cell lines and tissues in culture till half confluency. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• Properties
  MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  SDF-1β   Stromal   Derived   Factor   Beta   Kit  
• Gene symbol
  CXCL12, SFRP2, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
• Short name
  Mouse SDF-1β(Stromal Derived Factor 1 Beta) EIA Kit
• Technique
  Mouse, EIA, eia, mouses
• Host
  mouse
• Species
  Mouses
• Alternative name
  Mouse SDF-1β(Stromal cellular Derived Factor 1 b) EIA reagent
• Alternative technique
  murine, kits, elisas
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

Gene info

• Identity
  HGNC:10672
• Gene
  CXCL12
• Long gene name
  C-X-C motif chemokine ligand 12
• Synonyms gene
  • SDF1A
  • SDF1B
  • SDF1
• Synonyms gene name
  • stromal cell-derived factor 1
  • chemokine (C-X-C motif) ligand 12
• Synonyms
  • SCYB12
  • SDF-1a
  • SDF-1b
  • PBSF
  • TLSF-a
  • TLSF-b
  • TPAR1
• GenBank acession
  • L36033
• Locus
  10q11.21
• Discovery year
  1994-11-30
• Entrez gene record
  6387
• Pubmed identfication
  • 7490086
• RefSeq identity
  • NM_000609
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000018054

Gene info

• Identity
  HGNC:10777
• Gene
  SFRP2
• Long gene name
  secreted frizzled related protein 2
• Synonyms
  • SARP1
  • SDF-5
  • FRP-2
• GenBank acession
  • AF017986
• Locus
  4q31.3
• Discovery year
  1998-07-15
• Entrez gene record
  6423
• Pubmed identfication
  • 9391078
• Classification
  • Secreted frizzled-related proteins
• VEGA ID
  OTTHUMG00000161559

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31545
• Gene
  MIR16-1
• Long gene name
  microRNA 16-1
• Synonyms gene
  • MIRN16-1
• Synonyms
  • hsa-mir-16-1
• Locus
  13q14.2
• Discovery year
  2004-04-23
• Entrez gene record
  406950
• RefSeq identity
  • NR_029486
• Classification
  • MicroRNA MIR15/16 family

MeSH Data

• Name
  Immunoenzyme Techniques
• Concept
  Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
• Tree numbers
  • E05.478.566.350
  • E05.478.583.400
  • E05.601.470.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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