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ELISA s application
For quantitative measurement of mouse OPG in cell culture supernatant, serum and plasma EDTA .
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Gene tested
murine OPG
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ELISA standard curve
Concentration(pg/ml) + 0 + 93.7 + 187.5 + 375 + 750 + 1500 + 3000 + 6000 O.D. + 0.021 + 0.097 + 0.179 + 0.316 + 0.555 + 1.122 + 1.737 + 2.261
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ELISA kit detection limits
93.7pg/ml to 6000pg/ml
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ELISA s sensitivity
< 5pg/ml
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Target of this ELISA
Natural and recombinant mouse OPG
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);682;1537;3574;722;1647;3844 ELISA standard deviations 32.1;86.1;218;38.3;118.6;288.3 CV(%);4.7;5.6;6.1;5.3;7.2;7.5
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ELISA kit description
GENTAUR's mouse OPG ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for OPG was used as capure antibodies on the ELISA test plate. The internal standards (NSO, E22-L401) and your samples are measured with a biotin conjugated polyclonal from goat specific for OPG will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse OPG in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse OPG antibodies;1 ST0000-10;Lyophilized recombinant mouse OPG standard;10ng/tubex2 AR1107;Biotinylated anti- mouse OPG antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m mouse OPG standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(EDTA) to the ELISA plate. Keep in mind that each mouse OPG standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
20-25
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More gene info
Osteoprotegerin(OPG) is identical to osteoclastogenesis inhibitory factor(OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.
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Antigen
NSO, E22-L401
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
Tumor necrosis factor receptor superfamily member 11B
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Gene name
tumor necrosis factor receptor superfamily, member 11b
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Alternative
MGC29565;_x000D_MGC93568;_x000D_OCIF;_x000D__x000D__x000D_OPG;_x000D_Osteoclastogenesis inhibitory factor;_x000D_Osteoprotegerin;_x000D_TNFRSF 11B;_x000D_TNFRSF11B;_x000D_TR 1;_x000D_TR1;_x000D_TR11B_HUMAN;_x000D_Tumor necrosis factor receptor superfamily member 11B
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Gen protein identity
O08712
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Entrez nr
18383
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
TNFRSF11B
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Short name
Mouse OPG PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Host
mouse, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Mouse, Mouses
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Alternative name
Mouse OPG PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
murine, elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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