Mouse Monoclonal IgG2a Anti-CD54 (ICAM-1) [1304]
-
Catalog numberCD54-1
-
PricePlease ask
-
Size100 µg
-
-
AvailabilityPlease contact us to check the availability
-
ImmunogenCultivated human endothelial cells
-
SpecificityHuman CD54, the antibody detects an epitope in domain 1 of the 95 kDa transmembrane protein.
-
Species reactivityHuman
-
Product presentationAffinity purified, lyophilized antibody. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.
-
Product categoryPrimary Antibody
-
Antibody s raised inMouse
-
Antibody s isotypeIgG2a
-
Antibody s clone1304
-
Product is supplied aslyophilisate
-
Recommended concentration for use2 µg/ml (IHC-P)
-
Protein concentration0.1 mg lyophilized
-
Positive controlHuman Tonsil
-
PretreatmentFormaldehyde-fixed tissue slices are preferably pre-treated with proteinase K.
-
Tested applicationsIHC(C, P)
-
Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
-
Storage methodStore at 2-8°C
-
UniProt numberGo to UniProt/SwissProt
-
Litterature1. Albelda S.M., and Buck C.A. (1990) Integrins and other cell adhesion molecules. FASEB J. 4; 2868-2880. 2. Freudenthal P.S., and Steinmann R.M. (1990) The distinct surface of human blood dendritic cells, as observed after an improved isolation method. PNAS 87; 7698-7702. 3. Klickstein L.B., and Springer T.A. (1995) CD54 (ICAM-1)cluster report. In Leucocyte Typing V: White Cell Differentiation Antigens. S. Schlossman et al. eds.Oxford University Press pp.1548-1550.
-
Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolICAM1, ICAM3, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
-
Short nameResearch
-
TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
IsotypeIgG2a
-
SpeciesMouse, Mouses
-
Alternative nameCD54, ICAM-1
-
Alternative techniquemurine, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameintercellular adhesion molecule 1
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1989-04-24
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Ig-like cell adhesion molecule family
- Immunoglobulin like domain containing
- Receptor ligands
- CD molecules
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameintercellular adhesion molecule 3
-
Synonyms
-
Locus
-
Discovery year1994-05-19
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Ig-like cell adhesion molecule family
- Immunoglobulin like domain containing
- CD molecules
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1/206 family
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 8-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 7-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Immunoglobulin kappa (IGK) orphons
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1289-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1289 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 101-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR101 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 16-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR15/16 family
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data