Mouse Monoclonal IgG2a Anti-Alpha-Actin smooth muscle [1A4]

  • Catalog number
    AA500
  • Price
    Please ask
  • Size
    5 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Synthetic N-terminal decapeptide of smooth muscle Alpha-Actin
  • Specificity
    Alpha-Actin, smooth muscle
  • Species reactivity
    Human and other vertebrates
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
  • Product category
    Primary Antibody
  • Antibody s raised in
    Mouse
  • Antibody s isotype
    IgG2a
  • Antibody s clone
    1A4
  • Product is supplied as
    ready to use
  • Recommended concentration for use
    neat
  • Protein concentration
    N/A
  • Positive control
    Intestine, Appendix
  • Pretreatment
    No protease pre-treatment required, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).
  • Tested applications
    IHC(C, P)
  • Recommended secondary reagent
    We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
  • Storage method
    Store at 2-8°C
  • UniProt number
    P62736 (ACTA_HUMAN)
  • Litterature
    1. Skalli O, Ropraz P., Trzeciak A., Benzonana G., Gillessen D. Gabbiani G. (1986) A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103; 2787-2796 2. Skalli O., Schürch W., Seemayer T., Lagacé R., Montandon D., Pittet B., et al. (1989) Myofibroblasts from diverse pathologic settings are heterogenous in their content of actin isoforms and intermediate filament proteins. Lab Invest. 60; 275-285 3. Rønnov-Jessen L., Van Deurs B., Celis J.E., and Petersen O.W. (1990) Smooth muscle differentiation in cultured human breast gland stromal cells. Lab. Invest. 63; 532-543 4. Hasegawa T., Hirose T., Kudo E., Abe J., and Hizawa K. (1990) Cytosteletal characteristics of myofibroblasts in benign and reactive fibroblastic lesions. Virch. Arch. (A) Pathol. Anat. 416; 375-382
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    The Bioprime is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Short name
    Bioprime
  • Technique
    Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG2a
  • Species
    Mouse, Mouses
  • Alternative name
    Alpha-Actin, smooth muscle
  • Alternative technique
    murine, antibodies
  • Tissue
    muscle
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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