Mouse Monoclonal [clone N-GAD65] (IgG1) to Human GAD65 Antibody

• Catalog number
  GEN249171
• Price
  Please ask
• Size
  50ug

• Also known as
  GAD65
• Other names
  glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  GAD65
• Gene name synonims
  GAD2
• Other gene names
  GAD2; GAD2; GAD65; GAD65; GAD-65
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG1
• Clone
  N-GAD65
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  Human (Homo sapiens), Monkey, Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Specifically recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Protein G Purified
• Form Appearance
  PBS, 0.09% sodium azide
• Concentration
  1 miligram per 1 mililiter
• Storage and shipping
  For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
• Tested applications
  Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Radioimmunoassay (RIA)
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  Monoclonal   N-GAD65]   IgG1   GAD65  
• Gene symbol
  GAD2, GH1, POLM, NRAS, SDC3, PIGN, WASL, MAP6, HLA-N, FERD3L
• Short name
  Mouse Monoclonal [clone N-GAD65] (IgG1) GAD65 Antibody
• Technique
  Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Species
  Human, Humans, Mouses
• Alternative name
  Anti-Mouse Monoclonal [clone N-GAD65] (IgG1) to Human GAD65
• Alternative technique
  antibodies, murine
• Clone name
  N-GAD65

Gene info

• Identity
  HGNC:4093
• Gene
  GAD2
• Long gene name
  glutamate decarboxylase 2
• Synonyms gene name
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
• Synonyms
  • GAD65
• GenBank acession
  • AJ251501
• Locus
  10p12.1
• Discovery year
  1991-11-29
• Entrez gene record
  2572
• Pubmed identfication
  • 2039509
• RefSeq identity
  • NM_000818
• VEGA ID
  OTTHUMG00000017836

Gene info

• Identity
  HGNC:4261
• Gene
  GH1
• Long gene name
  growth hormone 1
• Synonyms
  • GH-N
  • GHN
  • GH
  • hGH-N
• Synonyms name
  • pituitary growth hormone
  • somatotropin
• GenBank acession
  • M13438
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  2688
• Pubmed identfication
  • 6306568
• RefSeq identity
  • NM_000515
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172293

Gene info

• Identity
  HGNC:9185
• Gene
  POLM
• Long gene name
  DNA polymerase mu
• Synonyms gene name
  • polymerase (DNA directed), mu
  • polymerase (DNA) mu
• Synonyms
  • Tdt-N
• Synonyms name
  • Pol iota
• GenBank acession
  • AF176097
• Locus
  7p13
• Discovery year
  2000-02-16
• Entrez gene record
  27434
• Pubmed identfication
  • 10982892
• RefSeq identity
  • NM_013284
• Classification
  • DNA polymerases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155353

Gene info

• Identity
  HGNC:7989
• Gene
  NRAS
• Long gene name
  NRAS proto-oncogene, GTPase
• Synonyms gene name
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • neuroblastoma RAS viral oncogene homolog
• Synonyms
  • N-ras
• GenBank acession
  • BC005219
• Locus
  1p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4893
• Pubmed identfication
  • 6705568
  • 11524732
• RefSeq identity
  • NM_002524
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000012059
• Locus Specific Databases
  • NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
  • LRG_92

Gene info

• Identity
  HGNC:10660
• Gene
  SDC3
• Long gene name
  syndecan 3
• Synonyms gene name
  • syndecan 3 (N-syndecan)
• Synonyms
  • N-syndecan
  • SYND3
• Synonyms name
  • syndecan proteoglycan 3
• GenBank acession
  • AF248634
• Locus
  1p35.2
• Discovery year
  1994-05-16
• Entrez gene record
  9672
• Pubmed identfication
  • 1556152
  • 11527150
• RefSeq identity
  • NM_014654
• Classification
  • Syndecans
• VEGA ID
  OTTHUMG00000043646

Gene info

• Identity
  HGNC:8967
• Gene
  PIGN
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class N
• Synonyms gene name
  • phosphatidylinositol glycan, class N
• Synonyms
  • MCD4
  • PIG-N
• GenBank acession
  • AF109219
• Locus
  18q21.33
• Discovery year
  2000-05-11
• Entrez gene record
  23556
• Pubmed identfication
  • 10069808
  • 10574991
• RefSeq identity
  • NM_176787
• Classification
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000180098

Gene info

• Identity
  HGNC:12735
• Gene
  WASL
• Long gene name
  WASP like actin nucleation promoting factor
• Synonyms gene name
  • Wiskott-Aldrich syndrome-like
  • Wiskott-Aldrich syndrome like
• Synonyms
  • N-WASP
  • NWASP
  • WASPB
• GenBank acession
  • D88460
• Locus
  7q31.32
• Discovery year
  1998-10-14
• Entrez gene record
  8976
• Pubmed identfication
  • 9422512
  • 9322739
  • 23527602
• RefSeq identity
  • NM_003941
• Classification
  • Wiskott-Aldrich Syndrome protein family
• VEGA ID
  OTTHUMG00000157346

Gene info

• Identity
  HGNC:6868
• Gene
  MAP6
• Long gene name
  microtubule associated protein 6
• Synonyms
  • KIAA1878
  • STOP
  • FLJ41346
  • MAP6-N
• GenBank acession
  • AK123340
• Locus
  11q13.5
• Discovery year
  1998-02-26
• Entrez gene record
  4135
• Pubmed identfication
  • 10516426
  • 12231625
• RefSeq identity
  • NM_033063
• VEGA ID
  OTTHUMG00000165343

Gene info

• Identity
  HGNC:19406
• Gene
  HLA-N
• Long gene name
  major histocompatibility complex, class I, N (pseudogene)
• Synonyms
  • HLA-30
• GenBank acession
  • M96329
• Locus
  6p22.1
• Discovery year
  2002-10-23
• Entrez gene record
  267014
• Pubmed identfication
  • 10452889
  • 1517563
• RefSeq identity
  • NG_009940
• Classification
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031167
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:16660
• Gene
  FERD3L
• Long gene name
  Fer3 like bHLH transcription factor
• Synonyms gene name
  • Fer3-like (Drosophila)
  • Fer3-like bHLH transcription factor
• Synonyms
  • NATO3
  • N-TWIST
  • bHLHa31
• GenBank acession
  • AF369897
• Locus
  7p21.1
• Discovery year
  2002-12-09
• Entrez gene record
  222894
• Pubmed identfication
  • 11472856
  • 12217327
• Classification
  • Basic helix-loop-helix proteins
• VEGA ID
  OTTHUMG00000090823

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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