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Also known as
ALPL / Alkaline Phosphatase
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Other names
alkaline phosphatase, tissue-nonspecific isozyme isoform 1; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
ALPL
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Gene name synonims
N/A
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Other gene names
ALPL; ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP; AP-TNAP; TNSALP
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
2F4
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Human TNAP / ALPL; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified
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Form Appearance
PBS, 0.05% sodium azide, 0.05% protein stabilizer
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
ALPL
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Short name
Mouse Monoclonal [clone 2F4] (IgG1) ALPL / Alkaline Phosphatase Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Label
Alkaline Phosphatase
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Species
Human, Humans, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone 2F4] (IgG1) to Human ALPL / Alkaline Phosphatase
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Alternative technique
antibodies, murine
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Alternative to gene target
alkaline phosphatase, liver/bone/kidney, AP-TNAP and APTNAP and HOPS and TNAP and TNSALP, ALPL and IDBG-93194 and ENSG00000162551 and 249, metal ion binding, Extracellular, Alpl and IDBG-199139 and ENSMUSG00000028766 and 11647, ALPL and IDBG-645614 and ENSBTAG00000008951 and 280994
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Clone name
2F4
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