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Availability
Please contact us to check the availability
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Immunogen
Purified Tenascin derived from mammary tumour cells
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Specificity
Human Tenascin, extracellular matrix protein
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Species reactivity
Human
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Product presentation
Purified antibody in phosphate buffer, BSA and 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
T2H5
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10-1:50
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Protein concentration
50 µg/ml
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Positive control
Colon carcinoma, abortive tissue, hyperproliferative skin, tonsil
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Pretreatment
Unmasking methods for paraffin sections are not established
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Tested applications
IHC(C,P), WB
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
P24821
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Litterature
1. Le Poole IC, van den Wijngaard RM, Westerhof W, Das PK. Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion. Br J Dermatol. 1997 Aug;137(2):171-8. 2. Le Poole IC, van den Wijngaard RM, Westerhof W, Das PK. Presence of T cells and macrophages in inflammatory vitiligo skin parallels melanocyte disappearance. Am J Pathol. 1996 Apr;148(4):1219-28. 3. Rulo HF, van Vlijmen-Willems IM, Schalkwijk J, Verstraeten AA, van de Kerkhof PC. Normal human skin demonstrates marked site-variation of tenascin expression, not correlated to epidermal proliferation (Ki-67 binding).J Dermatol Sci. 1993 Feb;5(1):54-7. 4. Verstraeten AA, Mackie EJ, Hageman PC, Hilgers J, Schol DJ, De Jongh GJ, Schalkwijk J. Tenascin expression in basal cell carcinoma. Br J Dermatol. 1992 Dec;127(6):571-4. 5.. Müller S., Neureiter D., Stolte M. et al. (1991) Virchows Arch. 438; 435-441.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
TNC
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Short name
ECM
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Technique
Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Mouse, Mouses
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Alternative name
Tenascin C (TNC)
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Alternative technique
murine, antibodies
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Alternative to gene target
tenascin C, 150-225 and DFNA56 and GMEM and GP and HXB and JI and TN and TN-C, TNC and IDBG-82348 and ENSG00000041982 and 3371, syndecan binding, Extracellular, Tnc and IDBG-155840 and ENSMUSG00000028364 and 21923, TNC and IDBG-638654 and ENSBTAG00000000575 and 540664
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