Mouse Monoclonal IgG1 Anti-Podoplanin (PDPN, M2A Antigen) (D2-40) [D2-40]

• Catalog number
  PDP001-0.5
• Price
  Please ask
• Size
  0.5 ml

• Availability
  Please contact us to check the availability
• Immunogen
  M2A Antigen, Podoplanin
• Specificity
  Podoplanin, M2A Antigen of lymphatic endothelia
• Species reactivity
  Human, other species not tested
• Product presentation
  Affinity purified antibody in PBS, BSA, NaN3
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG1
• Antibody s clone
  D2-40
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  1:30-1:100
• Protein concentration
  N/A
• Positive control
  Lymph nodes, Germ cell tumors
• Pretreatment
  The best pre-treatment of paraffin sections has not been validated at our lab. Meanwhile we suggest the usage of routine heat induced antigen retrieval although some laboratory results were positive without any unmasking.
• Tested applications
  IHC (C,P)
• Recommended secondary reagent
  Anti-mouse IgG:Biotin conjugate (code no. ZU102) in combination with Streptavidin-HRPO (code no. ZU054) and a suitable substrate e.g. DAB (Ref. DA002) or AEC (Ref AE002).
• Storage method
  Store at 2-8°C
• UniProt number
  Q86YL7 (PDPN_HUMAN)
• Litterature
  1. Hiroaki Yokomori Masaya Oda, Fumihiko Kaneko, Shigeyuki Kawachi, Minoru Tanabe, Kazunori Yoshimura, Yuko Kitagawa, and Toshifumi Hibi Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver- Re-evaluations of microlymphatic abnormalities Gastroenterology 2010, 10:131 2. Annette M. Müller, Folker E. Franke, Klaus-Michael Müller (2006) D2-40: A Reliable Marker in the Diagnosis of Pleural Mesothelioma Pathobiology 73:50-54 3. Yu H, Pinkus GS, Hornick JL. Diffuse membranous immunoreactivity for podoplanin (D2-40) distinguishes primary and metastatic seminomas from other germ cell tumors and metastatic neoplasms. Am J Clin Pathol. 2007 Nov;128(5):767-75. 4. Qingmei Xie, Lugen Chen, Kai Fu, Josephine Harter, Ken H Young, Jaya Sunkara, Deborah Novak, Esperanza Villanueva-Siles, and Howard Ratech (2008) Podoplanin (D2-40): A New Immunohistochemical Marker for Reactive Follicular Dendritic Cells and Follicular Dendritic Cell Sarcomas Int J Clin Exp Pathol. 1(3): 276–284. 5. Nelson G. Ordóñez (2005) D2-40 and podoplanin are highly specific and sensitive immunohistochemical markers of epithelioid malignant mesothelioma Human Pathol. 36(4) 372-380
• Tips
  Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Antigens are peptides or recombinant or native dependent on the production method.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG1   Podoplanin   PDPN   M2A   D2-40   [D2-40]  
• Gene symbol
  PDPN, NCAPD2, SNORD115-40, IGHV7-40, IGKV2D-40, IGHD2-15, PPID, IGKV2-40, IGLV1-40, IGHVII-40-1
• Short name
  Research
• Technique
  Mouse monoclonal, antigen, anti-, Mouse, anti, antibody to, antibodies, antigenes, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Species
  Mouse, Mouses
• Alternative name
  Podoplanin (PDPN, M2A Antigen) (D2-40)
• Alternative technique
  antigenes, murine, antibodies
• Alternative to gene target
  podoplanin, AGGRUS and GP36 and Gp38 and GP40 and HT1A-1 and OTS8 and PA2.26 and T1A and T1A-2 and T1A2 and TI1A, PDPN and IDBG-90582 and ENSG00000162493 and 10630, Cell surfaces, Pdpn and IDBG-202630 and ENSMUSG00000028583 and 14726, BOVAGGRUS and IDBG-636063 and ENSBTAG00000001788 and 509732

Gene info

• Identity
  HGNC:29602
• Gene
  PDPN
• Long gene name
  podoplanin
• Synonyms
  • T1A-2
  • Gp38
  • aggrus
  • GP40
  • PA2.26
  • D2-40
• Synonyms name
  • lung type I cell membrane associated glycoprotein
• GenBank acession
  • AB127958
  • AY194238
• Locus
  1p36.21
• Discovery year
  2005-07-08
• Entrez gene record
  10630
• Pubmed identfication
  • 10393083
  • 9651190
• RefSeq identity
  • NM_006474
• VEGA ID
  OTTHUMG00000007912

Gene info

• Identity
  HGNC:24305
• Gene
  NCAPD2
• Long gene name
  non-SMC condensin I complex subunit D2
• Synonyms gene name
  • non-SMC condensin I complex, subunit D2
• Synonyms
  • CNAP1
  • hCAP-D2
  • CAP-D2
  • KIAA0159
• Synonyms name
  • chromosome condensation related SMC associated protein 1
• GenBank acession
  • D63880
• Locus
  12p13.31
• Discovery year
  2006-09-04
• Entrez gene record
  9918
• Pubmed identfication
  • 8590280
  • 10958694
• RefSeq identity
  • NM_014865
• Classification
  • Condensin I subunits
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000168513

Gene info

• Identity
  HGNC:33059
• Gene
  SNORD115-40
• Long gene name
  small nucleolar RNA, C/D box 115-40
• Synonyms
  • HBII-52-40
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033814
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003355.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5666
• Gene
  IGHV7-40
• Long gene name
  immunoglobulin heavy variable 7-40 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable 7-40
  • immunoglobulin heavy variable 7-40 pseudogene
• GenBank acession
  • M99644
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28381
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152088

Gene info

• Identity
  HGNC:5804
• Gene
  IGKV2D-40
• Long gene name
  immunoglobulin kappa variable 2D-40
• GenBank acession
  • X59311
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28878
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000159963

Gene info

• Identity
  HGNC:5489
• Gene
  IGHD2-15
• Long gene name
  immunoglobulin heavy diversity 2-15
• Synonyms
  • IGHD215
  • D2
• GenBank acession
  • J00234
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28503
• Pubmed identfication
  • 7312051
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152430

Gene info

• Identity
  HGNC:9257
• Gene
  PPID
• Long gene name
  peptidylprolyl isomerase D
• Synonyms gene name
  • peptidylprolyl isomerase D (cyclophilin D)
• Synonyms
  • CYP-40
  • CypD
• Synonyms name
  • cyclophilin 40
• Locus
  4q32.1
• Discovery year
  1995-08-23
• Entrez gene record
  5481
• Pubmed identfication
  • 8509368
• RefSeq identity
  • NM_005038
• Classification
  • Cyclophilin peptidylprolyl isomerases
  • Tetratricopeptide repeat domain containing
• VEGA ID
  OTTHUMG00000161927

Gene info

• Identity
  HGNC:5789
• Gene
  IGKV2-40
• Long gene name
  immunoglobulin kappa variable 2-40
• GenBank acession
  • X59314
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28916
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000188265

Gene info

• Identity
  HGNC:5877
• Gene
  IGLV1-40
• Long gene name
  immunoglobulin lambda variable 1-40
• GenBank acession
  • M94116
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28825
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151072

Gene info

• Identity
  HGNC:5679
• Gene
  IGHVII-40-1
• Long gene name
  immunoglobulin heavy variable (II)-40-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-40-1
  • immunoglobulin heavy variable (II)-40-1 pseudogene
• Synonyms
  • IGHV(II)-40-1
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28368
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152098

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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