Mouse Monoclonal IgG1 Anti-NSE, Gamma-Enolase [MID-N3]

• Catalog number
  NS100
• Price
  Please ask
• Size
  1 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Purified human gamma-gamma-enolase
• Specificity
  Gamma-gamma isoform of enolase
• Species reactivity
  Human, guinea pig
• Product presentation
  Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG1
• Antibody s clone
  MID-N3
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  1:10-1:50
• Protein concentration
  50 µg/ml
• Positive control
  Adrenal medulla, nerve tissue
• Pretreatment
  No pre-treatment with proteases necessary, improvement of staining with the usage of unmasking fluid G (Art. No. DE007)
• Tested applications
  IHC(P)
• Recommended secondary reagent
  We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
• Storage method
  Store at 2-8°C
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  1. Marangos P.J., Schmechel D., Parma A.M., Clark R.L., and Goodwin F.K. (1979) Measurement of neuron-specific (NSE) and non-neuronal (NNE) isoenzymes of enolase in rat, monkey and human nervous tissue. J. Neurochem. 33; 319-329. 2. Cras P., Soler Federsppiel S., Gheuens J., Martin J.-J., Löwenthal A. (1986) Demonstration of neuron-specific enolase in nonneuronal tumors using a specific monoclonal antibody. Ann. Neurol. 20; 106-107. 3. Osborn M., Dirk T., Käser H., Weber K., and Altmannsberger M. (1986) Immunohistochemical localization of neurofilaments and neuron-specific enolase in 29 cases of neuroblastoma. Am. J. Pathol. 122; 433-442. 4. Cras P., Martin J.J., and Gheuens J. (1998) Gamma-enolase and glial fibrillary acidic protein in nervous system tumors. An immunohistochemical study using a specific monoclonal antibody. Acta Neuropathol. 75; 377-384.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG1   NSE   Gamma-Enolase   [MID-N3]  
• Gene symbol
  SPANXN3, CACNA2D1
• Short name
  Bioprime
• Technique
  Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Species
  Mouse, Mouses
• Alternative name
  NSE, Gamma-Enolase
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:33176
• Gene
  SPANXN3
• Long gene name
  SPANX family member N3
• Synonyms gene name
  • SPANX family, member N3
• Synonyms
  • SPANX-N3
  • CT11.8
• Synonyms name
  • cancer/testis antigen family 11, member 8
• Locus
  Xq27.3
• Discovery year
  2006-11-13
• Entrez gene record
  139067
• Pubmed identfication
  • 14973187
  • 17012309
• RefSeq identity
  • NM_001009609
• Classification
  • SPANX family
• VEGA ID
  OTTHUMG00000022582

Gene info

• Identity
  HGNC:1399
• Gene
  CACNA2D1
• Long gene name
  calcium voltage-gated channel auxiliary subunit alpha2delta 1
• Synonyms gene
  • CACNL2A
  • CACNA2
  • MHS3
  • LINC01112
• Synonyms gene name
  • long intergenic non-protein coding RNA 1112
  • calcium channel, voltage-dependent, alpha 2/delta subunit 1
• Synonyms
  • lncRNA-N3
• GenBank acession
  • M76559
• Locus
  7q21.11
• Discovery year
  1992-03-27
• Entrez gene record
  781
• Pubmed identfication
  • 8188232
• Classification
  • Calcium voltage-gated channel auxiliary alpha2delta subunits
• VEGA ID
  OTTHUMG00000023622
• Locus Specific Databases
  • LRG_437

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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