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Availability
Please contact us to check the availability
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Immunogen
Extract from lymph node metastases of melanoma
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Specificity
Neuraminidase-sensitive glycoconjugate on the surface of human melanosomes
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Species reactivity
Human
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
HMB45
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
N/A
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Positive control
Melanoma
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Pretreatment
Pre-treatment with pronase 0,1% or unmasking fluid G (Art. No. DE007)
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Tested applications
IHC(P)
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Recommended secondary reagent
We recommend the use of Gentaur'S Universal Staining System AEC (Art.-No. AE005), but also other sensitive staining systems are applicable (red staining usually is preferred).
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Gown A.M., et al., (1986) Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am. J. Pathol. 123; 195 ff. 2. Kapur R.P., et al. (1992) Anti-melanoma monoclonal antibody HMB45 identified an oncofetal glycoconjugate associated with immature melanosomes. J. Histochem Cytochem 40(2); 207 ff. 3. Leong A.S.-Y., and Millos J. (1989) An assessment of a melanoma-specific antibody HMB-45 and other immunohistochemical markers of malignant melanoma in paraffin-embedded tissues. Surg. Pathol. 2; 137 ff.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus