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Availability
Please contact us to check the availability
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Immunogen
Recombinant human oestrogen receptor alpha (ESR1)
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Specificity
N-terminal 67 kDa polypeptide of the human estrogen receptor alpha.
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Species reactivity
Human, rat
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for 200-500 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
1D5
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:20 - 1:50
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Protein concentration
40 µg/ml
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Positive control
Mamma carcinoma
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Pretreatment
Pre-treatment with Unmasking Fluid C (Citrate Buffer, Art. No. DE000) at 96-100 degree of Celsius is recommended for paraffin sections.
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Tested applications
IHC(P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Al Saati T., Clamens S., Cohen-Knafo E., Faye J.C., Prats H., Coindre J.M., et al. (1993) Production of monoclonal antibodies to human estrogen receptor protein (ER) using recombinant ER (RER). Int. J. Cancer 55; 651-654. 2. Sannino P. and Shousha S. (1994) Demonstration of oestrogen receptors in paraffin wax sections of breast carcinoma using the monoclonal antibody 1D5 and microwave oven processing. J. Clin. Pathol. 47; 90-92. 3. Mauri F.A., Veronese S., Frigo B., Girlando S., Losi L., Gambocorta M., et al. (1994) ER 1D5 and H222 (ER-ICA) antibodies to human estrogen receptor protein in breast carcinomas. Results of a multicentric comparative study. Appl. Immunohistochem. 2; 157-163. 4. Barnes D.M., Harris W.H., Smith P., Millis R.R., and Rubens R.D. (1996) Immunohistochemical determination of oestrogen receptor: comparison of different methods of assessment of staining and correlation with clinical outcome of breast cancer patients. Br. J. Cancer 74; 1445-1451.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
