Mouse Monoclonal IgG1 Anti-CEL (N-Epsilon)-Carboxyethyl-Lysine : Biotin conjugate [KNH-30]

• Catalog number
  CEL025BIO
• Price
  Please ask
• Size
  50 µg

• Availability
  Please contact us to check the availability
• Immunogen
  N-epsilon-(Carboxyethyl)-Lysine-BSA
• Species reactivity
  not relevant
• Product presentation
  Protein G affinity purified antibody from ascites in stabilized buffer, containing 50% Block Ace (Casein-containing solution, Dainippon Co.) and 0.1% ProClin (Rohm & Haas) as a preservative
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG1
• Antibody s clone
  KNH-30
• Product is supplied as
  liquid concentrate
• Recommended concentration for use
  ELISA 0.1-0.5 µg/ml; IHC 2 µg/ml
• Protein concentration
  0.25 mg/ml
• Positive control
  Diabetic lens cataract
• Tested applications
  IHC, ELISA
• Recommended secondary reagent
  Streptavidin:HRPO conjugate (Art. No. ZU054) or alkaline phosphatase conjugate (Art.-Nr. ZU051) in combination with a suitable substrate.
• Storage method
  Store at -20°C
• UniProt number
  Go to UniProt/SwissProt
• Litterature
  1. Horiuchi S., Araki N., and Morino Y. (1991) Immunological approach to characterize advanced glycation end products of the Maillard reaction: Evidence for the presence of a common stucture. J. Biol. Chem. 266; 7329-7332. 2. McLellan AC, Thornalley PJ, Benn J, Sonksen PH (1994) Glyoxalase system in clinical diabetes mellitus and correlation with diabetic complications. Clinical Science 87: 21-29. 3. Ahmed MU, Brinkmann E, Degenhardt TP, Thorpe SR, Baynes JW (1997) Ne-(Carboxyethyl)lysine, a product of the chemical modification of proteins by methylglyoxal, increases with age in human lens proteins. Biochem J 324:565-570. 4. Degenhardt TP, Thorpe SR, Baynes JW (1998) Chemical modification of proteins by methylglyoxal. Cell Mol Biol 44:1139-1145. 4. Degenhardt TP, Thorpe SR, Baynes JW (1998) Chemical modification of proteins by methylglyoxal. Cell Mol Biol 44:1139-1145.
• Tips
  Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG1   CEL   N-Epsilon   Carboxyethyl-Lysine   conjugate   [KNH-30]  
• Gene symbol
  CEL, SNORD114-30, SNORD116-30, SNORD115-30, IGKV2-30, RNU6-30P, IGHV3-30, IGLV3-30, IGHVII-30-21, IGHVII-30-1
• Short name
  AGE
• Technique
  Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Label
  Biotin
• Species
  Mouse, Mouses
• Alternative name
  CEL (N-Epsilon)-Carboxyethyl-Lysine : Biotin conjugate
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:1848
• Gene
  CEL
• Long gene name
  carboxyl ester lipase
• Synonyms
  • BSSL
  • MODY8
• Synonyms name
  • bile salt-stimulated lipase
• GenBank acession
  • M54994
• Locus
  9q34.13
• Discovery year
  1990-12-19
• Entrez gene record
  1056
• Pubmed identfication
  • 1676983
• Classification
  • Lipases
• VEGA ID
  OTTHUMG00000020855

Gene info

• Identity
  HGNC:33018
• Gene
  SNORD114-30
• Long gene name
  small nucleolar RNA, C/D box 114-30
• Synonyms
  • 14q(II-30)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767611
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003223.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:42797
• Gene
  SNORD116-30
• Long gene name
  small nucleolar RNA, C/D box 116-30
• Synonyms
  • HBII-85-30
• Locus
  15q11.2
• Discovery year
  2011-08-22
• Entrez gene record
  100873856
• RefSeq identity
  • NR_047032
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33049
• Gene
  SNORD115-30
• Long gene name
  small nucleolar RNA, C/D box 115-30
• Synonyms
  • HBII-52-30
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033804
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003345.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:5785
• Gene
  IGKV2-30
• Long gene name
  immunoglobulin kappa variable 2-30
• GenBank acession
  • X63403
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28919
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151692

Gene info

• Identity
  HGNC:34274
• Gene
  RNU6-30P
• Long gene name
  RNA, U6 small nuclear 30, pseudogene
• Synonyms gene
  • RNU6-30
• Synonyms gene name
  • RNA, U6 small nuclear 30
• Locus
  Xq22.1
• Discovery year
  2008-06-10
• Entrez gene record
  100873747
• RefSeq identity
  • NR_046490

Gene info

• Identity
  HGNC:5591
• Gene
  IGHV3-30
• Long gene name
  immunoglobulin heavy variable 3-30
• GenBank acession
  • M83134
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28439
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000188332

Gene info

• Identity
  HGNC:5912
• Gene
  IGLV3-30
• Long gene name
  immunoglobulin lambda variable 3-30 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable 3-30
  • immunoglobulin lambda variable 3-30 pseudogene
• GenBank acession
  • Z73646
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28789
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151182

Gene info

• Identity
  HGNC:52400
• Gene
  IGHVII-30-21
• Long gene name
  immunoglobulin heavy variable (II)-30-21 (pseudogene)
• Synonyms
  • IGHV(II)-30-21
• Locus
  14q32.33
• Discovery year
  2016-11-10
• Entrez gene record
  107548099
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33

Gene info

• Identity
  HGNC:5676
• Gene
  IGHVII-30-1
• Long gene name
  immunoglobulin heavy variable (II)-30-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-30-1
  • immunoglobulin heavy variable (II)-30-1 pseudogene
• Synonyms
  • IGHV(II)-30-1
• GenBank acession
  • AB019439
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28371
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152099

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products