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Availability
Please contact us to check the availability
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Immunogen
Monocyte preparation
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Specificity
Neutrophilic granulocytes, monocytes, and reactive macrophages
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Species reactivity
Human, monkey, cat, cattle, dog, guinea pig, horse, pig, rabbit, rat
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
MAC387
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Product is supplied as
ready to use
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Recommended concentration for use
neat
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Protein concentration
N/A
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Positive control
Spleen
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Pretreatment
Pre-treatment with pronase 0.1% (Art. No. DE110), Unmasking Fluid C (Art. No. DE000), or Unmasking Fluid G (Art. No. DE007)
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Tested applications
IHC(P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Jones D.B., Flavell D.J., Wright D.H. (1987) M4.12. Tissue reactivity of monoclonal antibody directed against cells of the human monocyte-macrophage lineage in formalin-fixed, paraffin-embedded tissue. In: McMichael A.J. et al. Eds. Leucocyte Typing III. White Cell Differentiation Antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 692-694. 2. Flavell D.J., Jones D.B., Wright D.H. (1987) Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. J. Histochem. Cytochem. 35; 1217-1226. 3. Steinbakk M., Naess-Andresen C.F., Lingaas E., Dale I., Brandtzaeg P., and Fagerhol M.K. (1990) Antimicrobial actions of calcium binding leucocyte L1 protein, calprotectin. Lancet 336; 763-765. 4. Brandtzaeg P., Dale I., Gabrielsen T.-O. (1992) The leucocyte protein L1 (calprotectin): usefulness as an immunohistochemical marker antigen and putative function. Histopathology 21; 191-196. 5. Goebeler M, Roth J, Teigelkamp S, Sorg C. The monoclonal antibody MAC387 detects an epitope on the calcium-binding protein MRP14. J Leukoc Biol. 1994 Feb;55(2):259-61.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus