Mouse Monoclonal IgG2b Anti-p53 Protein [DO-7]

  • Catalog number
    PP500
  • Price
    Please ask
  • Size
    5 ml
  • Availability
    Please contact us to check the availability
  • Immunogen
    Recombinant human p53 protein (wild type) produced in E. coli
  • Specificity
    N-terminal sequence of the human p53 protein, wild-type and mutant
  • Species reactivity
    Human
  • Product presentation
    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art. No. PU002, if further dilution is required.
  • Product category
    Primary Antibody
  • Antibody s raised in
    Mouse
  • Antibody s isotype
    IgG2b
  • Antibody s clone
    DO-7
  • Product is supplied as
    ready to use
  • Recommended concentration for use
    neat
  • Protein concentration
    N/A
  • Positive control
    Breast carcinoma
  • Pretreatment
    Pre-treatment with Unmasking Fluid G (Art. No. DE007) or citrate buffer (Art. No. DE000) at 96-100°C.
  • Tested applications
    IHC(P)
  • Recommended secondary reagent
    We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
  • Storage method
    Store at 2-8°C
  • UniProt number
    Go to UniProt/SwissProt
  • Litterature
    1. Cattoretti, G., Rilke F., Andreola S., D'Amato L., Delia D. (1988) p53 expression in breast cancer. Int. J. Cancer 41; 178-183. 2. Nigro M.J., Baker S.J., Preisinger A.C., Jessup J.M., Hostetter R., Cleary K., et al. (1989) Mutations in the p53 gene occur in divers human tumor types. Nature 342; 705-708. 3. Voitesek, Bartek J., Midgley C.A., Lane D.P. (1992) An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J. Immunol. Methods 151; 237-244. 4. Van den Berg F.M., Baas I.O., Polak M.M., Offerhaus G.J.A. (1993) Detection of p53 overexpression in routinely paraffin-embedded tissue of human carcinomas using a novel target unmasking fluid. Am. J. Pathol. 142; 381-385.
  • Tips
    *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    Monoclonal   IgG2b   p53   Protein   [DO-7]  
  • Gene symbol
    PIRC34, ART4, PIRC33, PIRC32, SNORD114-7, IGHV3OR15-7, SNORD115-7, RNU6-7, PIRC29, PIRC30
  • Short name
    Bioprime
  • Technique
    Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Isotype
    IgG2b
  • Species
    Mouse, Mouses
  • Alternative name
    p53 Protein
  • Alternative technique
    murine, antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 34
  • Locus
    7
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 33
  • Locus
    7
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 32
  • Locus
    7
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable 3/OR15-7 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable 3/OR15-7
    • immunoglobulin heavy variable 3/OR15-7 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • Classification
    • Immunoglobulin heavy (IGH) orphons
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 29
  • Locus
    7
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 30
  • Locus
    7
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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