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Also known as
MUC16 / CA125
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Other names
mucin-16; Mucin-16; N/A; N/A; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
MUC16
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Gene name synonims
CA125
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Other gene names
N/A; MUC16; N/A; CA125; MUC-16; CA-125
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
X75 (3C8/4)
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Specific for Mucin 16, also known as Ovarian Cancer Antigen CA-125. Recognizes epitope specificity group B (ISOBM classification) similar to M-11. Mucin 16 is a serum marker, overexpressed in epithelial ovarian cancer cells and is routinely used for patient monitoring. CA-125 was originally identified by Bast et al. using a monoclonal antibody developed against the ovarian cancer cell line OVCA433; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Ion exchange Chromatography
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Form Appearance
Tris buffered saline, 0.09% sodium azide.
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
MUC16, NBR1
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Short name
Mouse Monoclonal [clone X75 (3C8/4)] (IgG1) MUC16 / CA125 Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Human, Humans, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone X75 (3C8/4)] (IgG1) to Human MUC16 / CA125
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Alternative technique
antibodies, murine
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Alternative to gene target
mucin 16, cell surface associated, MUC16 and IDBG-25369 and ENSG00000181143 and 94025, Extracellular, Muc16 and IDBG-136368 and ENSMUSG00000032134 and 73732, MUC16 and IDBG-643029 and ENSBTAG00000037693 and 786607
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Clone name
X75
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