-
Also known as
EMA / MUC1
-
Other names
mucin-1 isoform 1; Mucin-1; mucin-1; episialin; DF3 antigen; H23 antigen; cancer antigen 15-3; krebs von den Lungen-6; mucin 1, transmembrane; carcinoma-associated mucin; polymorphic epithelial mucin; peanut-reactive urinary mucin; tumor associated epithelial mucin; breast carcinoma-associated antigen DF3; tumor-associated epithelial membrane antigen; Medullary cystic kidney disease, autosomal dominant; mucin 1, cell surface associated; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD_antigen: CD227Cleaved into the following 2 chains:Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-betaAlternative name(s):MUC1-CT
-
Category
Antibodies
-
Subcategory
Mnoclonal antibodies
-
Gene name
EMA
-
Gene name synonims
MUC1
-
Other gene names
MUC1; MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC; PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
-
Clonality
Monoclonal
-
Immunoglobulin isotype
IgG1
-
Clone
CM1
-
Host organism
Mouse (Mus musculus)
-
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivity
VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosylated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These MAbs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific MAbs and belong to MAb cluster 1-4 according to ISOMB TD-4 classification. No cross-reactivity with egg white avidin. MAbs M4H2, M2F1, M2C5, M2E104 and M3A106: Affinity >1 X 108 all MAbs. MAbs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conmations and peptide- carbohydrate compositions. MAb M4H2 reacts with VNTR20 recombinant unglycosylated fragment of MUC1 protein (and very weakly with monomeric MUC1 peptide), with deglycosylated and underglycosylated natural MUC1 (but not with completely glycosylated natural milk MUC1). MAbs M2F1, M2C5, M2E104, M3A106 efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. All these MAbs belong to MAb clusters 6 and 7 according to ISOMB TD-4 classification. Non cross-reactive with normal serum proteins and human tissues of non-epithelial origin.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification method
Protein A Purified
-
Form Appearance
PBS, pH 7.4, 0.1 % sodium azide.
-
Concentration
1 miligram per 1 mililiter
-
Storage and shipping
Store the antibody at +4 degrees Celsius. Freezing of the antibody is not recommended.
-
Tested applications
Immunohistochemistry (IHC - Paraffin, IHC - Frozen), Immunofluorescence (IF)
-
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
