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Also known as
SMAD3
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Other names
mothers against decapentaplegic homolog 3 isoform 1; Mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; SMAD family member 3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
SMAD3
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Gene name synonims
N/A
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Other gene names
SMAD3; SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
AF9F7
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Purified
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Form Appearance
HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
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Concentration
N/A
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Storage and shipping
For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
SMAD3
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Short name
Mouse Monoclonal [clone AF9F7] (IgG1 k) SMAD3 Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Human, Humans, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone AF9F7] (IgG1,k) to Human SMAD3
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Alternative technique
antibodies, murine
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Alternative to gene target
SMAD family member 3, HSPC193 and HsT17436 and JV15-2 and LDS1C and LDS3 and MADH3, SMAD3 and IDBG-18366 and ENSG00000166949 and 4088, transforming growth factor beta receptor, nuclei, Smad3 and IDBG-175693 and ENSMUSG00000032402 and 17127, SMAD3 and IDBG-645466 and ENSBTAG00000012599 and 515125
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Clone name
AF9F7
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