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Also known as
DNMT / DNMT1
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Other names
DNA (cytosine-5)-methyltransferase 1 isoform b; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
DNMT
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Gene name synonims
DNMT1
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Other gene names
DNMT1; DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
60B1220.1
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Host organism
Mouse (Mus musculus)
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Species reactivity
Bat, Gibbon, Gorilla, Horse, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus) Predicted Reactivity: Bovine, Dog, Hamster, Pig, Sheep (at least 90% immunogen sequence identity); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein G Purified
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Form Appearance
PBS, 0.05% BSA, 0.05% sodium azide.
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Concentration
0.5 mg/ml
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Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
