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Also known as
RELA / NFKB p65
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Other names
transcription factor p65 isoform 1; Transcription factor p65; transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel avian reticuloendotheliosis viral oncogene homolog A; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
RELA
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Gene name synonims
NFKB
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Other gene names
RELA; RELA; p65; NFKB3; NFKB3
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
112A1021
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Host organism
Mouse (Mus musculus)
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Species reactivity
Gibbon, Bovine, Chimpanzee, Dog, Gorilla, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Orangutan, Pig Predicted Reactivity: Bat, Horse (at least 90% immunogen sequence identity); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
A synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein. It will cross-react with mouse NF-kB p65.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein G Purified
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Form Appearance
PBS containing 0.05% BSA and 0.05% sodium azide. Sourced in TCS.
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Concentration
0.5 mg/ml
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Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
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French translation
anticorps
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Gene target
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Gene symbol
RELA-DT, RELA, NFKB1
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Short name
Mouse Monoclonal [clone 112A1021] (IgG1 k) RELA / NFKB p65 Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Human, Humans, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone 112A1021] (IgG1,k) to Human RELA / NFKB p65
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Alternative technique
antibodies, murine
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Alternative to gene target
v-rel reticuloendotheliosis viral oncogene homolog A (avian), NFKB3 and p65, RELA and IDBG-57543 and ENSG00000173039 and 5970, ankyrin repeat binding, nuclei, Rela and IDBG-132298 and ENSMUSG00000024927 and 19697, BT.26220 and IDBG-644222 and ENSBTAG00000013895 and 508233
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Clone name
112A1021
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