Anti-TIF1 gamma Antibody (3H7-C8-B9)
-
Catalog number
A1313-100
-
Price
Please ask
-
Size
100 µg
-
-
Antibody Target
TRIM33
-
Target Alternative Name
ECTO, PTC7, RFG7, TF1G, TIF1G, TIF gamma, TIF1gamma
-
Antibody Type
Monoclonal
-
Clone
3H7-C8-B9
-
Species Reactivities
Human
-
Immunogen Sequence
Recombinant human TIF1 gamma / TRIM33 protein fragments expressed in E.coli.
-
Accession
Q9UPN9
-
Gene Id
51592
-
Appearance
Colorless liquid
-
Form
Liquid
-
Formulation
In buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
-
Purification
Affinity purified
-
Application
WB
-
Positive Control
WB: MCF7 and HeLa nuclear extracts
-
Application And Usages
WB; 1:1000
-
Description
Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade)
-
Storage Condition
-20 °C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans
-
Handling
The antibody solution should be gently mixed before use
-
Western Blot Verified
Verified
-
Immunocytochemistry Verified
Not Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Not Verified
-
FACS Verified
Not Verified
-
ELISA Verified
Not Verified
-
ChIP Verified
Not Verified
-
Dot Blot Verified
Not Verified
-
Flow Cytometry Verified
Not Verified
-
-
Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
-
Gene target
-
Gene symbol
NDUFA3, B9D1, TRIM24
-
Short name
Anti-TIF1 gamma Antibody (3H7-C8-B9)
-
Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
-
Isotype
Mouse IgG2a
-
Alternative name
Antibody toTIF1 g (antibody to-) (3H7-C8-B9)
-
Alternative technique
antibodies
-
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
B9 domain containing 1
-
Synonyms gene name
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
2007-08-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- B9 domain containing
- MKS complex
- MicroRNA protein coding host genes
-
VEGA ID
-
Locus Specific Databases
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products