Anti-RAD9A Antibody (3A3-A7-F8)

• Catalog number
  A1316-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  RAD9A
• Target Alternative Name
  hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A, Cell cycle checkpoint control protein RAD9A
• Antibody Type
  Monoclonal
• Clone
  3A3-A7-F8
• Species Reactivities
  Human
• Immunogen Sequence
  Recombinant human RAD9A protein fragments expressed in E.coli
• Accession
  Q99638
• Gene Id
  5883
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS (pH 7.4) containing with 0.2% sodium azide, 50% glycerol
• Purification
  Affinity purified
• Application
  WB, IP
• Positive Control
  WB: HeLa, MCF7, 3T3, COS7 and Rat testis cell lysates; IP: HeLa and 3T3 cells
• Application And Usages
  WB; 1:500, IP
• Description
  Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  RAD9A   3A3-A7-F8  
• Gene symbol
  RAD9A, SNAR-A7, PCDHGA7, F8, TRERNA1
• Short name
  Anti-RAD9A Antibody (3A3-A7-F8)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG2b
• Alternative name
  Antibody toRAD9A (antibody to-) (3A3-A7-coagulation factor VIII, procoagulant component)
• Alternative technique
  antibodies
• Alternative to gene target
  RAD9 homolog A (S. pombe), RAD9, RAD9A and IDBG-60163 and ENSG00000172613 and 100130987,5883, histone deacetylase binding, nuclei, Rad9 and IDBG-129237 and ENSMUSG00000024824 and 19367, RAD9A and IDBG-644689 and ENSBTAG00000012141 and 504647

Gene info

• Identity
  HGNC:9827
• Gene
  RAD9A
• Long gene name
  RAD9 checkpoint clamp component A
• Synonyms gene
  • RAD9
• Synonyms gene name
  • RAD9 (S. pombe) homolog
  • RAD9 homolog A (S. pombe)
• GenBank acession
  • U53174
• Locus
  11q13.2
• Discovery year
  1997-02-03
• Entrez gene record
  5883
• Pubmed identfication
  • 8943031
  • 20081369
• RefSeq identity
  • NM_004584
• Classification
  • Checkpoint clamp complex
• VEGA ID
  OTTHUMG00000167670

Gene info

• Identity
  HGNC:34310
• Gene
  SNAR-A7
• Long gene name
  small NF90 (ILF3) associated RNA A7
• Synonyms gene name
  • small nuclear ILF3/NF90-associated RNA A7
  • small ILF3/NF90-associated RNA A7
• Synonyms
  • snaR-A55302203
• GenBank acession
  • EU071057
• Locus
  19q13.33
• Discovery year
  2008-06-20
• Entrez gene record
  100169953
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_024224
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:8705
• Gene
  PCDHGA7
• Long gene name
  protocadherin gamma subfamily A, 7
• Synonyms
  • PCDH-GAMMA-A7
• GenBank acession
  • AF152514
• Locus
  5q31
• Discovery year
  2000-06-28
• Entrez gene record
  56108
• Pubmed identfication
  • 10380929
• RefSeq identity
  • NM_018920
• Classification
  • Clustered protocadherins
• VEGA ID
  OTTHUMG00000163685

Gene info

• Identity
  HGNC:3546
• Gene
  F8
• Long gene name
  coagulation factor VIII
• Synonyms gene
  • F8C
• Synonyms gene name
  • coagulation factor VIII, procoagulant component
• Synonyms
  • FVIII
  • DXS1253E
  • HEMA
• Synonyms name
  • Factor VIIIF8B
  • hemophilia A
• GenBank acession
  • M90707
• Locus
  Xq28
• Discovery year
  2001-06-22
• Entrez gene record
  2157
• Pubmed identfication
  • 6438528
  • 3935400
• VEGA ID
  OTTHUMG00000022688
• Locus Specific Databases
  • HAMSTeRS: The Haemophilia A Mutation, Structure, Test & Resource Site
  • Global Variome shared LOVD
  • LRG_555

Gene info

• Identity
  HGNC:44307
• Gene
  TRERNA1
• Long gene name
  translation regulatory long non-coding RNA 1
• Synonyms gene
  • LINC00651
• Synonyms gene name
  • long intergenic non-protein coding RNA 651
• Synonyms
  • ncRNA-a7
  • treRNA
• Synonyms name
  • translational regulatory long non-coding RNA
• GenBank acession
  • AI968488
  • BX118093
• Locus
  20q13.13
• Discovery year
  2012-08-09
• Entrez gene record
  100887755
• Pubmed identfication
  • 20887892
  • 23974796
• RefSeq identity
  • NR_051976
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000032711

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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