Anti-Mouse I-Ab (private; Ia.m20), Biotin (Clone 25-5-16S) (mouse IgM)

• Catalog number
  GEN520472
• Price
  Please ask
• Size
  100ug

• Also known as
  I-Ab (private; Ia.m20)
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  Mouse IgM
• Clone
  25-5-16S
• Host organism
  Mouse (Mus musculus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  I-Ab (Mouse I-Ab); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  N/A
• Form Appearance
  Biotin
• Concentration
  Antibody Concentration: 0.1 miligram per 1 mililiter
• Storage and shipping
  Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles. Check label for expiry date.
• Tested applications
  Immunohistochemistry (IHC)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
• Conjugation
  Biotinylated
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  I-Ab   private   m20   25-5-16S  
• Gene symbol
  SNORD116-25, ZNF211, SNORD114-25, SNORD115-25, ERVW-25, IGKV3D-25, IGLV3-25, RNVU1-25, IGKV3-25, IGHVIII-25-1
• Short name
  Anti-Mouse I-Ab (private; Ia m20), (Clone 25-5-16S) (mouse IgM)
• Technique
  anti-, Mouse, anti, IgM, antibody to, antibodies, IgMs, mouses
• Host
  mouse
• Isotype
  IgM, IgM
• Label
  Biotin
• Species
  Mouse, Mouses
• Alternative name
  Anti-Anti-Mouse I-Ab (private; Ia.m20), Biotin (Clone 25-5-16S) (mouse IgM)
• Alternative technique
  murine, antibodies, immunoglobulin-m
• Clone name
  25-5-16S

Gene info

• Identity
  HGNC:33091
• Gene
  SNORD116-25
• Long gene name
  small nucleolar RNA, C/D box 116-25
• Synonyms
  • HBII-85-25
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033436
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003339
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:13003
• Gene
  ZNF211
• Long gene name
  zinc finger protein 211
• Synonyms
  • ZNF-25
  • CH2H2-25
• GenBank acession
  • U38904
• Locus
  19q13.4
• Discovery year
  1999-09-29
• Pubmed identfication
  • 7633419
  • 9096115
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000168012

Gene info

• Identity
  HGNC:33013
• Gene
  SNORD114-25
• Long gene name
  small nucleolar RNA, C/D box 114-25
• Synonyms
  • 14q(II-25)
• Locus
  14q32.31
• Discovery year
  2006-09-26
• Entrez gene record
  767605
• Pubmed identfication
  • 12045206
• RefSeq identity
  • NR_003218.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:33044
• Gene
  SNORD115-25
• Long gene name
  small nucleolar RNA, C/D box 115-25
• Synonyms
  • HBII-52-25
• GenBank acession
  • AF250841
• Locus
  15q11.2
• Discovery year
  2007-02-15
• Entrez gene record
  100033801
• Pubmed identfication
  • 11106375
  • 11726556
• RefSeq identity
  • NR_003342.1
• Classification
  • Small nucleolar RNAs, C/D box

Gene info

• Identity
  HGNC:49313
• Gene
  ERVW-25
• Long gene name
  endogenous retrovirus group W member 25
• Synonyms gene name
  • endogenous retrovirus group W, member 25
• GenBank acession
  • HG421063
• Locus
  13q13.3
• Discovery year
  2013-10-21

Gene info

• Identity
  HGNC:5826
• Gene
  IGKV3D-25
• Long gene name
  immunoglobulin kappa variable 3D-25 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 3D-25
  • immunoglobulin kappa variable 3D-25 pseudogene
• GenBank acession
  • X71886
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28873
• RefSeq identity
  • NG_000833
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151609

Gene info

• Identity
  HGNC:5908
• Gene
  IGLV3-25
• Long gene name
  immunoglobulin lambda variable 3-25
• GenBank acession
  • X97474
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28793
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151221

Gene info

• Identity
  HGNC:54428
• Gene
  RNVU1-25
• Long gene name
  RNA, variant U1 small nuclear 25
• Locus
  1q21.1
• Discovery year
  2019-07-26
• Classification
  • Variant U1 small nuclear RNAs

Gene info

• Identity
  HGNC:5818
• Gene
  IGKV3-25
• Long gene name
  immunoglobulin kappa variable 3-25 (pseudogene)
• Synonyms gene name
  • immunoglobulin kappa variable 3-25
  • immunoglobulin kappa variable 3-25 pseudogene
• GenBank acession
  • X06583
• Locus
  2p11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28911
• RefSeq identity
  • NG_000834
• Classification
  • Immunoglobulin kappa locus at 2p11.2
• VEGA ID
  OTTHUMG00000151645

Gene info

• Identity
  HGNC:5697
• Gene
  IGHVIII-25-1
• Long gene name
  immunoglobulin heavy variable (III)-25-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (III)-25-1
  • immunoglobulin heavy variable (III)-25-1 pseudogene
• Synonyms
  • IGHV(III)-25-1
• GenBank acession
  • AB019439
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28348
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152072

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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