Mouse IgG2b Isotype Control, FITC Conjugated mAb

• Catalog number
  SAB-28279
• Price
  Please ask
• Size
  50 Tests

• Application
  FC IF
• Species Reactivity
  Hu
• Source
  Mouse monoclonal Ab
• Storage
  Store at -20°C
• Category
  Flow Cytometric Ab
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This SAB Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. SAB FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• Group
  isotypes
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  IgG2b   Conjugated   mAb  
• Short name
  Mouse IgG2b Isotype Control, FITC Conjugated mAb
• Technique
  Control, Isotype, Mouse, FITC, antibody Conjugates, controls, Fluorescein, Isotypes and controls, mouses
• Host
  mouse
• Isotype
  IgG2b
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  Mouse IgG2b isoform reference, fluorecein coupled monoclonal
• Alternative technique
  control, controls, murine, fluorescine
• Tissue
  control

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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