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Category
Serums
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Long description
This standard is prepared by pooling serum obtained from a large number mice belonging to a-allotype and b-allotype stains with the following assigned values: IgG1--2.00 mg/ml. IgG2a--8.54 mg/ml. IgG2b--1.25 mg/ml. IgG3--0.754 mg/ml. IgA--2.75 mg/m. IgM--0.277 mg/ml. The determination of the concentration of each of the four subclasses was carried out by single radial immunodiffusion (RID, Mancini) in agar gel Nordic Nr. 2 using subclass-specific polyclonal antisera and purified mouse immunoglobulins as primary standards. Allotypic differences in mouse IgG2a may interfere with the determination of this subclass. At least eight allelic variants of IgG2a have been identified. Most laboratory mouse strains fall into one of these allogeneic groups. Mouse Standard Serum NOR-02 contains IgG2a of both allelic types Igh-1aand Igh1b and cab be used for the quantitative determination of IgG2a in the majority of mouse strains, including Balb/C, C3H, CBA/J, SJL/J and SM/J. Mouse standard serum NOR-02 is intended for use as a calibrator to prepare standard curves in quantitative determinations of the immunoglobulins IgA and IgM and IgG subclasses.
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Antibody come from
n/a
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Other description
Delipidated, lyophilized serum. No preservative added
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Clone
not specified
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Antigen antibody binding interaction
Mouse Ig and IgG subclasses standard Serum
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Antibody is raised in
see techfile
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Antibody s reacts with
Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
Mouse standard Serum with assigned values for IgA, IgM and IgG subclasses.
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Research interest
Immunology
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Application
not tested yet / to be tested
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Antibody s suited for
The product is shipped lyophilized at ambient temperature. The lyophilized content is reconstituted by adding 0.5 ml sterile water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted serum may be divided into small aliquots, frozen at or below -20°C. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS) containing 0.05% Tween and 1% (w/v) bovine serum albumin, should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
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Storage
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
