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ELISA s application
For quantitative measurement of rat soluble L-Selectin in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
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Gene tested
Rat sL-Selectin
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ELISA standard curve
Concentration(pg/ml) + 0 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 + 40000 O.D. + 0.004 + 0.550 + 0.919 + 1.411 + 1.799 + 2.074 + 2.129 + 2.226
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ELISA kit detection limits
625pg/ml to 40000pg/ml
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ELISA s sensitivity
< 20pg/ml
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Target of this ELISA
Natural and recombinant rat sL-Selectin
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Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
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ELISA s precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);5.3;12;29;8.8;16.5;31.4 ELISA standard deviations 0.18;0.516;1.653;0.449;1.023;2.17 CV(%);3.4;4.3;5.7;5.1;6.2;6.9
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ELISA kit description
GENTAUR's rat L-Selectin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibodies from mouse specific for L-Selectin was used as capure antibodies on the ELISA test plate. The internal standards (NSO, W39-N332) and your samples are measured with a biotin conjugated polyclonal from goat specific for L-Selectin is added subsequently and then followed by washing with PBS or TBS buffers. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffers. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat L-Selectin in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
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Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
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The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- rat L-Selectin antibodies;1 ST0000-40;Lyophilized recombinant rat L-Selectin standard;40ng/tube×2 AR1107;Biotinylated anti- rat L-Selectin antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
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Anti coagulation
heparin or EDTA
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How the elisa works
Sample the amount of 100ul/well of the standard solution at 40000pg/ml, 20000pg/ml,10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml rat L-Selectin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each rat L-Selectin standard solution and each sample be measured in duplicate.
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Minutes of TMB incubation
20-25
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More gene info
L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL(L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
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Antigen
NSO, W39-N332
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Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Uniprot
L-selectin
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Gene name
selectin L
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Alternative
A.11;AI528707;CD62 antigen ligand;CD62 antigen-like family member L;gp90 MEL;hLHRc;IgA nephropathy, susceptibility to, included L Selectin;LAM1;LECAM1;Leu8;Leukocyte adhesion molecule 1;Leukocyte surface antigen Leu8;Leukocyte-endothelial cell adhesion molecule 1;LNHR;LSEL;Ly-22;Ly-m22;LYAM1;Lymph node homing receptor;Lymphocyte adhesion molecule 1;Lymphocyte antigen 22;Lymphocyte surface MEL-14 antigen;PLNHR;Selectin L;Selectin, lymphocyte;SELL;TQ1
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Gen protein identity
P30836
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Entrez nr
29259
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
SELL
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Short name
sL-Selectin PicoKine ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
Rat, antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Rat, Rats
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Alternative name
Rat sL-Selectin PicoKine Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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