Mouse CD146 Human Monoclonal Antibody [TEA1/34]-Fitc
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Catalog number
BD-146F-100T
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Price
Please ask
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Size
100 TESTS
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Description
Mouse CD146 Human Monoclonal Antibody [TEA1/34]-Fitc is a human antibody, specific for [TEA1/34] clones, it links with CD146 antigens in Melanom, epithelial, and endothelial cells , also in activated T cells, multipotent mesenchymal stromal cells, CD146 plays a role of adhesion and monocytes transendothelial migration, this product was conjugated with Fitc format and reported for use in flow cytometric analysis.
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Conjugate
Fitc
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Gene name
CD146
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Other gene names
S-Endo, MUC18
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Clone
TEA1/34
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Application
Flow Cytometry Method [FCM].
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Expiration
after 1 year
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Storage
2-8°C, store in dark, do not freeze
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Properties
If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
MCAM
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Short name
Mouse CD146 Monoclonal Antibody [TEA1/34]-Fitc
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Technique
Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses
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Host
mouse
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Isotype
IgG2a
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Label
FITC
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Species
Human, Humans, Mouses
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Alternative name
Mouse CD146 H. sapiens monoclonal (antibody to-) [TEA1/34]-fluorecein
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Alternative technique
antibodies, murine, fluorescine
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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