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Clone
2A7.H6
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Immunogen
Full length protein HSP40 (YDJ1)
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Antibody s full description
Mouse Anti-Yeast HSP40 Monoclonal IgG1 Kappa Antibody, Clone: 2A7.H6: ATTO 700
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
DnaJ (HSP40) homolog subfmaily B member 1 antibody, DNAJ 1 antibody, DNAJ B1 antibody, DnaJ homolog subfamily B member 1 antibody, DnaJ protein homolog 1 antibody, DNAJ1 antibody, DNAJB 1 antibody, DNAJB1 antibody, DNAJB1 protein antibody, DNJB1_HUMAN antibody, HDJ 1 antibody, HDJ-1 antibody, HDJ1 antibody, Heat shock 40 kDa protein 1 antibody, Heat shock 40kD protein 1 antibody, Heat shock protein 40 antibody, HSP 40 antibody, HSP40 antibody, HSPF 1 antibody, HSPF1 antibody, Human DnaJ protein 1 antibody, Radial spoke 16 homolog B antibody, RSPH16B antibody, Sis1 antibody
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Raised in
Mouse
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Antibody s target
HSP40
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Primary research fields
Cancer, Heat Shock
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Brandname
None
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Antibodies applications
WB, IP, ELISA
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Antibody s reactivity
Yeast
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Antibody s dilutions
WB (1:2000); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~40kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
0.5 µg/ml of SMC-166 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm , Nucleus
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
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Bibliography
1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91. 4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890. 7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
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Released date
1-Nov-2008
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_014335.1
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Gene number
855661
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Protein number
P25491
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Antibody s datasheet
Contact our support service
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Representative figure legend
Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6 (SMC-166). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-166) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp40 Antibody [2A7.H6] used in Western Blot (WB) on Yeast recombinant cell lysate (SMC-166)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
