Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 1G10.H8

  • Catalog number
    SMC-150D-A594
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    1G10.H8
  • Immunogen
    Full length protein yeast HSP40 (YDJ1)
  • Antibody s full description
    Mouse Anti-Yeast HSP40 Monoclonal IgG1 Kappa Antibody, Clone: 1G10.H8: ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    DNAJA2 Antibody, CPR3 Antibody, HIRIP4 Antibody, DNAJ Antibody, DNJ3 Antibody, DJ3 Antibody, RDJ2 Antibody, HIRa interacting protein4 Antibody
  • Raised in
    Mouse
  • Antibody s target
    HSP40
  • Primary research fields
    Cancer, Heat Shock
  • Brandname
    None
  • Antibodies applications
    WB, IP, ELISA
  • Antibody s reactivity
    Yeast
  • Antibody s dilutions
    WB (1:2000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~40kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Nucleus
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
  • Bibliography
    1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36. 2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316. 3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095. 4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830. 5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878. 6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931. 7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.
  • Released date
    1-Nov-2008
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_014335.1
  • Gene number
    855661
  • Protein number
    P25491
  • Antibody s datasheet
    Contact our support service
  • Representative figure legend
    Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8 (SMC-150). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (SMC-150) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Hsp40 Antibody [1G10.H8] used in Western Blot (WB) on Yeast Cell lysates (SMC-150)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
    Yeast   HSP40   YDJ1   Monoclonal   IgG1   Kappa   1G10  
  • Gene symbol
    DNAJB1
  • Short name
    Mouse Anti-Yeast HSP40, YDJ1 Monoclonal IgG1 Kappa, Clone 1G10 H8
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse, Ascomycota
  • Isotype
    IgG1 Kappa, IgG1
  • Label
    ATTO 594
  • Species
    Yeast, Mouses, Yeasts
  • Alternative name
    Mouse Antibody toYeast HSP40, YDJ1 monoclonal IgG1 Kappa, clonality 1G10.H8
  • Alternative technique
    murine, antibodies
  • Clone name
    1G10
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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