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Clone
2E7
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Immunogen
Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
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Antibody s full description
Mouse Anti-Yeast HDEL Monoclonal IgG2b Antibody, Clone: 2E7: ATTO 680
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody
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Raised in
Mouse
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Antibody s target
HDEL
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Primary research fields
Cancer, Heat Shock, Cell Signaling, Trafficking
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Brandname
None
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Antibodies applications
WB, ICC/IF
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Antibody s reactivity
Fruit Fly (Drosophila melanogaster), Yeast, Yeast (Saccharomyces cerevisiae), Plant, Barnyard Grass (Echinochloa), Beet (Beta vulgaris), Cotton (Gossypium), Mung Bean (Vigna radiata), Grass (Sorghum), Wheat (Triticum spp.)
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Antibody s dilutions
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~78kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Endoplasmic Reticulum
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
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Bibliography
1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.
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Released date
1-Apr-2010
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
Refer to NCBI
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Gene number
Refer to GenBank
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Protein number
Refer to Swiss-Prot
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Antibody s datasheet
Contact our support service
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
