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Category
Primary Antibodies
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Long description
Tumor Necrosis Factor alpha is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation._x000B_Genetic variations in TNF both influence susceptibility to hepatitis B virus (HBV) infection and are involved in susceptibility to malaria. Additionally, genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; and arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
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Antibody come from
Human recombinant tumor necrosis factor alpha (hTNF ).
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
AS3
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Antigen antibody binding interaction
Mouse anti Tumor Necrosis Factor alpha (TNF )* Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human, Baboon
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunoprecipitation, Enzyme Immunoassay
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Antibody s suited for
ELISA: This antibody has been used as a coating antibody at 5µg/ml in a two site ELISA with biotin conjugated TNF antibody (Cat. No. L190M). Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-500 µl. Recovery of hTNF in human serum is typically >85% in the ELISA. IC: This antibody has been used to immunoprecipitate > 90% TNF in solution using a double-antibody method (Midgley and Hepburn).
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Storage
-20ºC
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Relevant references
Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
The Mouse anti Tumor Necrosis Factor alpha (TNF )* is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
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Gene target
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Gene symbol
TNF, TNFRSF1A, TNFRSF1B, LTBR
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Short name
Mouse anti Tumor Necrosis Factor alpha (TNF )*
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Technique
Mouse, anti, antibody to, mouses
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Host
mouse
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Isotype
IgG2a
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Label
unlabeled
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Tumor Necrosis Factor a (tumor necrosis factor )*
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Alternative technique
murine, antibodies
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Alternative to gene target
tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
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Tissue
tumor
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