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Category
Primary Antibodies
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Long description
Transferrin receptor protein 1 (TfR1) also known as CD71 (Cluster of Differentiation 71) is a protein that in Humans is encoded by the TFRC gene. CD71 plays a critical role in cell prolifeRation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin, the major iron-carrying protein. The expression of CD71 is regulated at the post-transcriptional level through the control of mRNA stability and is closely linked to intracellular iron levels. Nitric oxide can affect CD71 expression independently of intracellular iron concentRation by activating proteins which then stabilize CD71 mRNA. Expression of CD71, or the transferrin receptor, is very low on resting leukocytes but is upregulated upon activation reflecting the iron dependence of activation. In other tissues, CD71 is expressed on most dividing cells and also on brain endothelium and alveolar macrophage. The ligand is the Serum iron transport protein transferrin. CD71 antibodies are used as an indiCator of prolifeRation in the typing of lymphomas and leukemias.
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Antibody come from
66IG10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with Human T-cells.
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Other description
Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
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Clone
66IG10
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Antigen antibody binding interaction
Mouse anti Transferrin Receptor (CD71) Antibody
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Antibody is raised in
Mouse (Balb/c)
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
66IG10, raised anti Human thymocytes (T-cells) was found to be directed anti the Human transferrin receptor. This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond.
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Research interest
Immunology, CD Markers
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Application
Flow Cytometry,Immunohistochemistry (frozen),Immunoprecipitation,Western blotting
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Antibody s suited for
66IG10 is useful for western blot (on non reduced antigens) and immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
K. Moolenaar et al. Cancer research 50,1102-1106, Feb 15 1990. Van de Rijna M, Geurts van Kessel AHM, Kroezen V, van Agthoven AJ, Verstijnen K, Terhorst C, Hilgers J: Cytogenet Cell Genet 1983;36:525-531 (DOI: 10.1159/000131967).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
