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Category
Primary Antibodies
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Long description
Thymidylate Synthase (TS)1 (EC 2.1.1.45) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal,4,5 gastric,5 head and neck,6 and breast7 carcinomas.
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Antibody come from
Synthetic peptide derived from the N terminal of the EDG3 (S1P3) protein.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
TS106
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Antigen antibody binding interaction
Mouse anti Thymidylate Synthase Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunoprecipitation, Immunohistochemistry (frozen & paraffin), Immunofluorescense, Enzyme Immunoassay, Flow Cytometry
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Antibody s suited for
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development.
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Storage
-20ºC
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Relevant references
1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998: 12, 1589-98._x000B__x000B_2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998: 30-31, 147-57.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
