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Category
Second Step Reagents
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Long description
Monoclonal Anti-Goat/Sheep IgG - Peroxidase Conjugate is is derived from the GT-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with goat IgG conjugated to purified horseradish peroxidase. Purified mouse immunoglobulin is conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde. The conjugated antibody is lyophilized from M phosphate buffered saline containing 0.01 1% BSA and 0.01% thimerosal as preservative._x000B_Peroxidase Monoclonal Anti-Goat/Sheep IgG recognizes an epitope located on the heavy chain of both goat IgG1 and IgG2. The product shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components, or with IgG derived from the following species: human, guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat.
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Antibody come from
n/a
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Other description
Provided as solution in phosphate buffered saline, pH 7.3, with 1% BSA and 0.1% 2-chloroacetamide.
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Clone
GT-34
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Antigen antibody binding interaction
Mouse anti sheep and goat HRP Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Goat, Sheep, Bovine
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, ELISA
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Antibody s suited for
Antibody can be used for the localization of goat or sheep IgG by ELISA, immunobotting and immunohistochemistry._x000B__x000B_Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
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Storage
-20ºC
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Relevant references
1. Artman, M., et al., K ATP channels of primary human coronary artery endothelial cells consist of a heteromultimeric complex of Kir6.1, Kir6.2, and SUR2B subunits. J. Mol. Cell. Cardiol. 37, 857-869, (2004) Abstract_x000B_ _x000B_2. Vicente, C.P., et al., Heparin cofactor II inhibits arterial thrombosis after endothelial injury J. Clin. Invest. 109, 213-219, (2002) Abstract_x000B_ _x000B_3. B’r, A., et al., Studies on the interactions between C-reactive protein and complement proteins Immunology 121, 40-50, (2007) Abstract_x000B_ _x000B_4. van Beekum, O., et al., The Adipogenic Acetyltransferase Tip60 Targets Activation Function 1 Of Peroxisome Proliferator-activated Receptor Gamma. Endocrinology 149, 1840-9, (2008) Abstract_x000B_ _x000B_5. Oksala, N., et al., ADAM-9, ADAM-15, And ADAM-17 Are Upregulated In Macrophages In Advanced Human Atherosclerotic Plaques In Aorta And Carotid And Femoral ArteriesÑTampere Ann. Med. 41, 279-90, (2009) Abstract
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Latin name
Capra aegagrus hircus, Mus musculus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Short name
Mouse anti goat
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Technique
Mouse, anti, Goat, antibody to, goats, mouses
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Host
mouse, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG1
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Label
HRP
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Species
Sheep, Goats, Mouses, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Mouse antibody to ovine and caprine horseradish peroxidase
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Alternative technique
murine, antibodies, caprine
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