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Category
Primary Antibodies
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Long description
Red Fluorescent Protein is thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts._x000B__x000B_Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
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Antibody come from
Synthetic peptide derived from the human PHLPP1 protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
RFD5R
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Antigen antibody binding interaction
Mouse anti RFP Tag (Red Fluorescent Protein) Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
WB, Dot Blt, ELISA,IP, IS
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Antibody s suited for
Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Gao, T., et al. ÔPHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growthÕ Mol. Cell, 18, 13-24, 2005_x000B__x000B_2. Bragnard, J., et al. ÔPHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms.Õ Mol. Cell, 25, 917-931, 2007_x000B__x000B_3. Gao, T., et al. ÔThe phosphatase PHLPP controls the cellular levels of protein kinase C.Õ J. Biol. Chem., 283, 6300-6311, 2008
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Conjugation
red dye
