Mouse Anti-Rat TrpV3 Monoclonal IgG2a, Clone S15-4

  • Catalog number
    SMC-319D-A633
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S15-4
  • Immunogen
    Synthetic peptide amino acids 774-791 (C-terminus) of rat TrpV3
  • Antibody s full description
    Mouse Anti-Rat TRPV3 Monoclonal IgG2a Antibody, Clone: S15-4: ATTO 633
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    1110036I10Rik Antibody, MGC124324 Antibody, MGC124325 Antibody, Transient receptor potential cation channel subfamily V member 3 Antibody, TrpV3 Antibody, Trpv3 heat sensitive channel Antibody, TRPV3_HUMAN Antibody, Vanilloid receptor 3 Antibody, Vanilloid receptor like 3 Antibody, Vanilloid receptor-like 3 Antibody, vanilloid receptor-related osmotically activated channel protein Antibody, VRL 3,VRL-3 Antibody, VRL3 Antibody
  • Raised in
    Mouse
  • Antibody s target
    TRPV3
  • Primary research fields
    Neuroscience, Ion Channels, Transient Receptor Potential Channels
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF, IP, AM
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH7.4, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~70kDa.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-319 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    The TRPV3 protein belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels (1, 2).
  • Bibliography
    1. Masamoto Y., Kawabata F., Fushiki T. (2009) Biosci. Biotechnol. Biochem. 73(5): 1021-1027. 2. Xiao R., et al. (2008) J Biol Chem. 283(10): 6162-6174.
  • Released date
    6-Jul-2009
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_001020928
  • Gene number
    497948
  • Protein number
    Q4QYD9
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-4 (SMC-319). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (SMC-319) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-4 (SMC-319). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (SMC-319) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-4 (SMC-319). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (SMC-319) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Mouse Anti-TrpV3 Antibody [S15-4] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-319) Mouse Anti-TrpV3 Antibody [S15-4] used in Western Blot (WB) on Human Cell lysates (SMC-319) Mouse Anti-TrpV3 Antibody [S15-4] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-319)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
    TrpV3   Monoclonal   IgG2a   S15-4  
  • Gene symbol
    RPS15, MIR1302-4, PIRC18, PIRC16, SNORD114-4, IGKV2OR2-4, MIR941-4, PIRC19
  • Short name
    Mouse Anti- TrpV3 Monoclonal IgG2a, Clone S15-4
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2a, IgG2a
  • Label
    ATTO 633
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat TrpV3 monoclonal IgG2a, clonality S15-4
  • Alternative technique
    murine, antibodies
  • Clone name
    S15-4
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 18
  • Locus
    4
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 16
  • Locus
    4
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin kappa variable 2/OR2-4 (pseudogene)
  • Synonyms gene name
    • immunoglobulin kappa variable 2/OR2-4
    • immunoglobulin kappa variable 2/OR2-4 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-18
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Immunoglobulin kappa (IGK) orphons
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 19
  • Locus
    4
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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