Mouse Anti-Rat TASK1 Potassium Channel Monoclonal IgG2b, Clone S374-48

  • Catalog number
    SMC-473S
  • Price
    Please ask
  • Size
    12 µg
  • Clone
    S374-48
  • Immunogen
    Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical).
  • Antibody s full description
    Mouse Anti-Rat KCNK3 Monoclonal IgG2b Antibody, Clone: S374-48: Unconjugated
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Potassium channel subfamily K member 3 Antibody, KCNK3 Antibody, Acid sensitive potassium channel protein TASK 1 Antibody, Cardiac two pore background K(+) channel Antibody, cTBAK 1 Antibody, K2p3.1 Antibody, KCNK9 Antibody, OAT1 Antibody, potassium channel subfamily K member 3 Antibody, rTASK Antibody, TASK 1 Antibody, TBAK1 Antibody, TWIK related acid sensitive K+ channel Antibody, Two pore potassium channel KT3.1 Antibody, Two pore K(+) channel KT3.1 Antibody
  • Raised in
    Mouse
  • Antibody s target
    KCNK3
  • Primary research fields
    Neuroscience, Ion Channels, Potassium Channels, Tandem Pore Domain Potassium Channels
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~50kDa. Does not cross-react with TASK3.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-473 was sufficient for detection of TASK1 Potassium Channel in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Membrane
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54% identity with TASK-1, but less than 30% identity with TASK-2 and other tandem pore K+ channels.
  • Released date
    29-Oct-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_203694.1
  • Gene number
    29553
  • Protein number
    O54912
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (SMC-473). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (SMC-473) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) TASK1 Potassium Channel Antibody (D) Composite. Western Blot analysis of Rat Brain Membrane showing detection of ~50 kDa TASK1 Potassium Channel protein using Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody, Clone S374-48 (SMC-473). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TASK1 Potassium Channel Monoclonal Antibody (SMC-473) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~50 kDa. Mouse Anti-TASK1 Potassium Channel Antibody [S374-48] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-473) Mouse Anti-TASK1 Potassium Channel Antibody [S374-48] used in Western Blot (WB) on Rat Brain Membrane (SMC-473)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.012
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
  • Gene symbol
    SNORD115-48, IGLV5-48, IGHV3-48, UTP18, RNU6-48P, KCNK3, HAUS8, SNORD115-45
  • Short name
    Mouse Anti- TASK1 Potassium Channel Monoclonal IgG2b, Clone S374-48
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2b, IgG2b
  • Label
    Unconjugated
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat TASK1 Potassium Channel monoclonal IgG2b, clonality S374-48
  • Alternative technique
    murine, antibodies
  • Clone name
    S374-48
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin lambda variable 5-48 (non-functional)
  • Synonyms gene name
    • immunoglobulin lambda variable 5-48
  • GenBank acession
  • Locus
  • Discovery year
    2000-05-08
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin lambda locus at 22q11.2
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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