Mouse Anti-Rat Kv3.1 Monoclonal IgG1, Clone S16B-8
-
Catalog numberSMC-313D-P594
-
PricePlease ask
-
Size100 µg
-
-
CloneS16B-8
-
ImmunogenFusion protein amino acids 437-585 (C-terminus) or rat Kv3.1b
-
Antibody s full descriptionMouse Anti-Rat Kv3.1 Monoclonal IgG1 Antibody, Clone: S16B-8: PE/ATTO 594
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameKrc2 1 Antibody, NGK2 Antibody, KCNC1 Antibody, voltage gated potassium channel subunit Kv3.1 Antibody, Kv4 Antibody
-
Raised inMouse
-
Antibody s targetKv3.1
-
Primary research fieldsNeuroscience, Ion Channels, Potassium Channels, Voltage-Gated Potassium Channels
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF, AM
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~110kDa. Weakly reactive in human samples.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-313 was sufficient for detection of Kv3.1b in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellMembrane
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextPotassium voltage-gated channel, Shaw-related subfamily, member 1, also known as KCNC1 or Kv3.1, is a human gene. The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily (1). The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Kv3.1b has been extensively tested in the auditory regions of mammals, and the decline of Kv3.1b expression appears to correlate with the functional decline in the medial olivocochlear efferent system (2). Other research shows potential for Kv3.1b channels to be oxygen sensors (3).
-
Bibliography1. Xu M., et al. (2007) J. Neuroscience. 27(51): 14158-14170. 2. Zettel M.L., Zhu X., O’Neill W.E., Frisina R.D. (2007) J Assoc Res Otolaryngol. 8(2): 280-293. 3. Osipenko O.N., Tate R.J. and Gurney A.M. (2000) Circ Res. 86(5): 534-540.
-
Released date6-Jul-2009
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_036988
-
Gene number25327
-
Protein numberP25122
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunohistochemistry analysis using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 (SMC-313). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody (SMC-313) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Western Blot analysis of Rat brain lysates showing detection of Kv3.1 Potassium Channel protein using Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody, Clone S16B-8 (SMC-313). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv3.1 Potassium Channel Monoclonal Antibody (SMC-313) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-Kv3.1 Potassium Channel Antibody [S16B-8] used in Immunohistochemistry (IHC) on Human hippocampus (SMC-313) Mouse Anti-Kv3.1 Potassium Channel Antibody [S16B-8] used in Western Blot (WB) on Rat brain lysates (SMC-313)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus, Rattus norvegicus
-
Gene target
-
Gene symbolSNORD114-8, RNU6-8, SNORD113-8, SNORD116-8, IGKV2OR2-8, MIR1302-8, PIRC35
-
Short nameMouse Anti- Kv3 1 Monoclonal IgG1, Clone S16B-8
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG1, IgG1
-
LabelPE/ATTO 594
-
SpeciesRat, Mouses, Rats
-
Alternative nameMouse Antibody toRat Kv3.1 monoclonal IgG1, clonality S16B-8
-
Alternative techniquemurine, antibodies
-
Clone nameS16B-8
-
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 114-8
-
Synonyms
-
Locus
-
Discovery year2006-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameRNA, U6 small nuclear 8
-
Synonyms
-
Locus
-
Discovery year2008-06-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nuclear RNAs
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 113-8
-
Synonyms
-
Locus
-
Discovery year2006-09-20
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene namesmall nucleolar RNA, C/D box 116-8
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2007-02-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small nucleolar RNAs, C/D box
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 2/OR2-8 (pseudogene)
-
Synonyms gene name
- immunoglobulin kappa variable 2/OR2-8
- immunoglobulin kappa variable 2/OR2-8 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1302-8
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1302 family
Gene info
-
Identity
-
Gene
-
Long gene namepiwi-interacting RNA cluster 35
-
Locus
-
Discovery year2009-11-05
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Piwi-interacting RNA clusters
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
