Mouse Anti-Rat SHANK1/SHANK3 Monoclonal IgG2a, Clone S367-51

  • Catalog number
    SMC-460D-P594
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    S367-51
  • Immunogen
    Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
  • Antibody s full description
    Mouse Anti-Rat SHANK3 Monoclonal IgG2a Antibody, Clone: S367-51: PE/ATTO 594
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Shank postsynaptic density protein Antibody, SH3 and multiple ankyrin repeat domains 3 Antibody, Proline rich synapse associated protein 2 Antibody, Proline-rich synapse-associated protein 2 Antibody, ProSAP2 Antibody, PSAP2 Antibody, Antibody, SH3 and multiple ankyrin repeat domains protein 3 Antibody, SH3/ankyrin domain gene 3,Shank3b Antibody, SPANK 2 Antibody, SPANK2 Antibody, KAP/SAPAP interacting protein Antibody, OTTHUMP00000174437 Antibody, SH3 and multiple ankyrin repeat domains 1 Antibody, SH3 and multiple ankyrin repeat domains protein 1 Antibody, SH3/ankyrin domain gene 1 Antibody, SHANK 1 Antibody, Shank1 Antibody, Shank1a Antibody, Somatostatin receptor interacting protein Antibody, Somatostatin receptor-interacting protein Antibody, SPANK 1 Antibody, SPANK1 Antibody, SSTR interacting protein Antibody, SSTR-interacting protein Antibody, SSTRIP Antibody, Synamon Antibody, AI841104 Antibody, DEL22q13.3 Antibody, KIAA1650 Antibody
  • Raised in
    Mouse
  • Antibody s target
    SHANK3
  • Primary research fields
    Neuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Scaffolds
  • Brandname
    None
  • Antibodies applications
    WB, IHC, ICC/IF
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s dilutions
    WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.1% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    1 µg/ml of SMC-460 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm , Cell Junction
  • Tissue specificity
    See included datasheet or contact our support service
  • Scientific context
    SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.
  • Released date
    29-Oct-2013
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    NP_067708.1.
  • Gene number
    59312
  • Protein number
    Q9JLU4
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (SMC-460). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (SMC-460) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SHANK1/SHANK3 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (SMC-460). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (SMC-460) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa. Mouse Anti-SHANK1/SHANK3 Antibody [S367-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-460) Mouse Anti-SHANK1/SHANK3 Antibody [S367-51] used in Western Blot (WB) on Monkey COS cells transfected with HA-tagged Shank1 (SMC-460)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • Gene target
  • Gene symbol
    IGHV8-51-1, IGHVII-51-2, IGLV1-51, IGHV5-51, SHANK1, SHANK3, ZNF3, SAMM50, OR7E26P
  • Short name
    Mouse Anti- SHANK1/SHANK3 Monoclonal IgG2a, Clone S367-51
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG2a, IgG2a
  • Label
    PE/ATTO 594
  • Species
    Rat, Mouses, Rats
  • Alternative name
    Mouse Antibody toRat SHANK1/SHANK3 monoclonal IgG2a, clonality S367-51
  • Alternative technique
    murine, antibodies
  • Clone name
    S367-51
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable IGHV8-51-1 (non-functional)
  • Synonyms gene
  • Synonyms gene name
    • immunoglobulin heavy variable (III)-51-1
    • immunoglobulin heavy variable (III)-51-1 pseudogene
    • immunoglobulin heavy variable (III)-51-1 (pseudogene)
    • immunoglobulin heavy variable IGHV8-51-1 (pseudogene)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    immunoglobulin heavy variable (II)-51-2 (pseudogene)
  • Synonyms gene name
    • immunoglobulin heavy variable (II)-51-2
    • immunoglobulin heavy variable (II)-51-2 pseudogene
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2000-04-17
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Immunoglobulin heavy locus at 14q32.33
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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