Mouse Anti-Rat SHANK1/SHANK3 Monoclonal IgG2a, Clone S367-51
-
Catalog numberSMC-460D-P594
-
PricePlease ask
-
Size100 µg
-
-
CloneS367-51
-
ImmunogenFusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
-
Antibody s full descriptionMouse Anti-Rat SHANK3 Monoclonal IgG2a Antibody, Clone: S367-51: PE/ATTO 594
-
Antibody s categoryMonoclonal Antibodies
-
Antibody s other nameShank postsynaptic density protein Antibody, SH3 and multiple ankyrin repeat domains 3 Antibody, Proline rich synapse associated protein 2 Antibody, Proline-rich synapse-associated protein 2 Antibody, ProSAP2 Antibody, PSAP2 Antibody, Antibody, SH3 and multiple ankyrin repeat domains protein 3 Antibody, SH3/ankyrin domain gene 3,Shank3b Antibody, SPANK 2 Antibody, SPANK2 Antibody, KAP/SAPAP interacting protein Antibody, OTTHUMP00000174437 Antibody, SH3 and multiple ankyrin repeat domains 1 Antibody, SH3 and multiple ankyrin repeat domains protein 1 Antibody, SH3/ankyrin domain gene 1 Antibody, SHANK 1 Antibody, Shank1 Antibody, Shank1a Antibody, Somatostatin receptor interacting protein Antibody, Somatostatin receptor-interacting protein Antibody, SPANK 1 Antibody, SPANK1 Antibody, SSTR interacting protein Antibody, SSTR-interacting protein Antibody, SSTRIP Antibody, Synamon Antibody, AI841104 Antibody, DEL22q13.3 Antibody, KIAA1650 Antibody
-
Raised inMouse
-
Antibody s targetSHANK3
-
Primary research fieldsNeuroscience, Cell Structure, Post-Synaptic Markers, Cell Signaling, Scaffolds
-
BrandnameNone
-
Antibodies applicationsWB, IHC, ICC/IF
-
Antibody s reactivityHuman, Mouse, Rat
-
Antibody s dilutionsWB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
-
PurityProtein G Purified
-
Antibody buffer for storagePBS pH 7.4, 50% glycerol, 0.1% sodium azide
-
Antibody s concentration1 mg/ml
-
Antibody s specificityDetects ~190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
-
Storage recommendations-20ºC
-
Shipping recommendationsBlue Ice or 4ºC
-
Antibody certificate of analysis1 µg/ml of SMC-460 was sufficient for detection of SHANK1/SHANK3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cellCytoplasm , Cell Junction
-
Tissue specificitySee included datasheet or contact our support service
-
Scientific contextSHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.
-
Released date29-Oct-2013
-
Tested applicationsTo be tested
-
Tested reactivityTo be tested
-
NCBI numberNP_067708.1.
-
Gene number59312
-
Protein numberQ9JLU4
-
Antibody s datasheetContact our support service
-
Representative figure link
-
Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (SMC-460). Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (SMC-460) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SHANK1/SHANK3 Antibody (D) Composite. Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (SMC-460). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (SMC-460) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa. Mouse Anti-SHANK1/SHANK3 Antibody [S367-51] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line SK-N-BE (SMC-460) Mouse Anti-SHANK1/SHANK3 Antibody [S367-51] used in Western Blot (WB) on Monkey COS cells transfected with HA-tagged Shank1 (SMC-460)
-
Warning informationNon-hazardous
-
Country of productionCanada
-
Total weight kg1.4
-
Net weight g0.1
-
Stock availabilityIn Stock
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus, Rattus norvegicus
-
Gene target
-
Gene symbolIGHV8-51-1, IGHVII-51-2, IGLV1-51, IGHV5-51, SHANK1, SHANK3, ZNF3, SAMM50, OR7E26P
-
Short nameMouse Anti- SHANK1/SHANK3 Monoclonal IgG2a, Clone S367-51
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
-
Hostmouse
-
IsotypeIgG2a, IgG2a
-
LabelPE/ATTO 594
-
SpeciesRat, Mouses, Rats
-
Alternative nameMouse Antibody toRat SHANK1/SHANK3 monoclonal IgG2a, clonality S367-51
-
Alternative techniquemurine, antibodies
-
Clone nameS367-51
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable IGHV8-51-1 (non-functional)
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin heavy variable (III)-51-1
- immunoglobulin heavy variable (III)-51-1 pseudogene
- immunoglobulin heavy variable (III)-51-1 (pseudogene)
- immunoglobulin heavy variable IGHV8-51-1 (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-51-2 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-51-2
- immunoglobulin heavy variable (II)-51-2 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 1-51
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 5-51
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameSH3 and multiple ankyrin repeat domains 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2002-02-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
- PDZ domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameSH3 and multiple ankyrin repeat domains 3
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2002-02-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- PDZ domain containing
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namezinc finger protein 3
-
Synonyms gene name
- zinc finger protein 3 (A8-51)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1989-05-31
-
Entrez gene record
-
RefSeq identity
-
Classification
- Zinc fingers C2H2-type
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameSAMM50 sorting and assembly machinery component
-
Synonyms gene name
- sorting and assembly machinery component 50 homolog (S. cerevisiae)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2005-05-31
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Mitochondrial sorting and assembly machinery complex
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameolfactory receptor family 7 subfamily E member 26 pseudogene
-
Synonyms gene
-
Synonyms gene name
- olfactory receptor, family 7, subfamily E, member 26 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-11-29
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Olfactory receptors, family 7
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data